5S rDNA and U2 snRNA are linked in the genome of Crassostrea angulata and Crassostrea gigas oysters: does the (CT) n ·(GA) n microsatellite stabilize this novel linkage of large tandem arrays?

The 5S rRNA genes from 2 species of the Ostreidae family, Crassostrea angulata and Crassostrea gigas, were molecularly characterized. The genes were amplified, cloned, and sequenced. The results revealed a 5S rDNA tandem array with a nucleotide sequence in an inverted position within the nontranscri...

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Bibliographic Details
Published in:Genome
Main Authors: Cross, I, Rebordinos, L
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 2005
Subjects:
Genetics
Molecular Biology
General Medicine
Biotechnology
Crassostrea gigas
Online Access:http://dx.doi.org/10.1139/g05-075
http://www.nrcresearchpress.com/doi/pdf/10.1139/g05-075
Description
Summary:The 5S rRNA genes from 2 species of the Ostreidae family, Crassostrea angulata and Crassostrea gigas, were molecularly characterized. The genes were amplified, cloned, and sequenced. The results revealed a 5S rDNA tandem array with a nucleotide sequence in an inverted position within the nontranscribed spacer region that corresponded to the U2 small nuclear RNA (snRNA) gene. The sequence analysis indicated that both genes could be functionally active. The presence of the microsatellite (CT) n ·(GA) n at the 3′ end of both genes and the possible involvement of concerted evolution are discussed.Key words: Crassostrea angulata, Crassostrea gigas, 5S rDNA, U2 snRNA, microsatellite, concerted evolution.

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