Synaptonemal complex analysis in spermatocytes and oocytes of turbot, Scophthalmus maximus (Pisces, Scophthalmidae)

A surface-spreading synaptonemal complex (SC) technique was used to analyze spermatocytes and oocytes of turbot (Scophthalmus maximus) to visualize the process of chromosome synapsis. The total SC length was 205 ± 12 µm in males and 172 ± 29 µm in the only female analyzed. A representative SC karyot...

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Bibliographic Details
Published in:Genome
Main Authors: Cuñado, N, Terrones, J, Sánchez, L, Martínez, P, Santos, J L
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 2001
Subjects:
Genetics
Molecular Biology
General Medicine
Biotechnology
Scophthalmus maximus
Turbot
Online Access:http://dx.doi.org/10.1139/g01-104
http://www.nrcresearchpress.com/doi/pdf/10.1139/g01-104
Description
Summary:A surface-spreading synaptonemal complex (SC) technique was used to analyze spermatocytes and oocytes of turbot (Scophthalmus maximus) to visualize the process of chromosome synapsis. The total SC length was 205 ± 12 µm in males and 172 ± 29 µm in the only female analyzed. A representative SC karyotype of turbot was obtained. Each SC showed lateral elements of equal length. No bivalent exhibiting atypical synaptic behaviour that could be associated with heteromorphic sex chromosomes was observed, either in males or in the female. The DNA content of turbot was evaluated in eight individuals of both sexes by flow cytometry analysis. The 2C mean DNA content of turbot (1.308 ± 0.009 pg/cell) was among the lowest observed within fishes. No statistical differences in DNA content were revealed between the sexes [Wilcoxon/Mann–Whitney test; P(W x = 0.243)]. The SC/DNA content ratio observed in turbot was the highest reported to date in bony fishes (Osteichthyes).Key words: Scophthalmus maximus, fish, DNA content, meiosis, synaptonemal complex.

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