In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults

Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2...

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Published in:Canadian Journal of Fisheries and Aquatic Sciences
Main Authors: Likely, C. G., Burt, M. D. B.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1992
Subjects:
Gadus morhua
Online Access:http://dx.doi.org/10.1139/f92-039
http://www.nrcresearchpress.com/doi/pdf/10.1139/f92-039
id crcansciencepubl:10.1139/f92-039
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spelling crcansciencepubl:10.1139/f92-039 2024-09-15T18:07:16+00:00 In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults Likely, C. G. Burt, M. D. B. 1992 http://dx.doi.org/10.1139/f92-039 http://www.nrcresearchpress.com/doi/pdf/10.1139/f92-039 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 49, issue 2, page 347-348 ISSN 0706-652X 1205-7533 journal-article 1992 crcansciencepubl https://doi.org/10.1139/f92-039 2024-07-04T04:10:02Z Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2 and the culture was maintained at 35 °C. The mature nematodes proved to be Contracaecum osculatum, a species which normally matures in the stomach of pinnipeds. Article in Journal/Newspaper Gadus morhua Canadian Science Publishing Canadian Journal of Fisheries and Aquatic Sciences 49 2 347 348
institution Open Polar
collection Canadian Science Publishing
op_collection_id crcansciencepubl
language English
description Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2 and the culture was maintained at 35 °C. The mature nematodes proved to be Contracaecum osculatum, a species which normally matures in the stomach of pinnipeds.
format Article in Journal/Newspaper
author Likely, C. G.
Burt, M. D. B.
spellingShingle Likely, C. G.
Burt, M. D. B.
In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
author_facet Likely, C. G.
Burt, M. D. B.
author_sort Likely, C. G.
title In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
title_short In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
title_full In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
title_fullStr In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
title_full_unstemmed In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
title_sort in vitro cultivation of contracaecum osculatum (nematoda:anisakidae) from third-stage larvae to egg-laying adults
publisher Canadian Science Publishing
publishDate 1992
url http://dx.doi.org/10.1139/f92-039
http://www.nrcresearchpress.com/doi/pdf/10.1139/f92-039
genre Gadus morhua
genre_facet Gadus morhua
op_source Canadian Journal of Fisheries and Aquatic Sciences
volume 49, issue 2, page 347-348
ISSN 0706-652X 1205-7533
op_rights http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining
op_doi https://doi.org/10.1139/f92-039
container_title Canadian Journal of Fisheries and Aquatic Sciences
container_volume 49
container_issue 2
container_start_page 347
op_container_end_page 348
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