In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults

Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2...

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Bibliographic Details
Published in:Canadian Journal of Fisheries and Aquatic Sciences
Main Authors: Likely, C. G., Burt, M. D. B.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1992
Subjects:
Aquatic Science
Ecology, Evolution, Behavior and Systematics
Fildes
Gadus morhua
Online Access:http://dx.doi.org/10.1139/f92-039
http://www.nrcresearchpress.com/doi/pdf/10.1139/f92-039
Description
Summary:Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2 and the culture was maintained at 35 °C. The mature nematodes proved to be Contracaecum osculatum, a species which normally matures in the stomach of pinnipeds.

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