In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults
Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Canadian Science Publishing
1992
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Subjects: | |
Online Access: | http://dx.doi.org/10.1139/f92-039 http://www.nrcresearchpress.com/doi/pdf/10.1139/f92-039 |
Summary: | Third-stage (L 3 ) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N 2 – 10% CO 2 – 5% O 2 and the culture was maintained at 35 °C. The mature nematodes proved to be Contracaecum osculatum, a species which normally matures in the stomach of pinnipeds. |
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