Use of Erythrocyte Measurements to Identify Triploid Salmonids
Triploid landlocked Atlantic salmon (Salmo salar) were identified using microspectrophotometry to measure the DNA content of Feulgen-stained erythrocytes. Blood cell sizing by means of a Coulter Counter Channelyzer was a highly effective alternative for screening triploids, comparable with flow cyto...
| Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Canadian Science Publishing
1984
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1139/f84-112 http://www.nrcresearchpress.com/doi/pdf/10.1139/f84-112 |
| Summary: | Triploid landlocked Atlantic salmon (Salmo salar) were identified using microspectrophotometry to measure the DNA content of Feulgen-stained erythrocytes. Blood cell sizing by means of a Coulter Counter Channelyzer was a highly effective alternative for screening triploids, comparable with flow cytometry in speed and accuracy. The statistical analysis of various erythrocyte dimensions measured from blood smears was also a valid method for identifying triploids. No mosaic polyploids were found, and the existence of such fish is questioned. |
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