A Technique for Prolonged Blood Sampling in Free-Swimming Salmon
A technique is described for insertion of a cannula in the dorsal aorta of salmon for long-term blood sampling or vascular injection while the fish is confined, but free-swimming. Previous methods for single injections into the dorsal aorta are improved by a modified cannulation technique. The pract...
| Published in: | Journal of the Fisheries Research Board of Canada |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Canadian Science Publishing
1964
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1139/f64-064 http://www.nrcresearchpress.com/doi/pdf/10.1139/f64-064 |
| Summary: | A technique is described for insertion of a cannula in the dorsal aorta of salmon for long-term blood sampling or vascular injection while the fish is confined, but free-swimming. Previous methods for single injections into the dorsal aorta are improved by a modified cannulation technique. The practicality of the technique was tested by introducing Evans Blue (T-1824) into the dorsal aorta of immature pink salmon (Oncorhynchus gorbuscha) in sea water and mature sockeye salmon (O. nerka) in fresh water to make preliminary estimates of blood volumes. It was shown that the technique can also be applied to angiography of salmon. |
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