Glucose, fructose, and sucrose influx into Nitella flexilis

The uptake of 14 C-labeled glucose, fructose, and sucrose into Nitella flexilis has been investigated by varying concentrations of sugars, by the use of competition experiments, by the use of inhibitors, and by varying conditions of temperature. The kinetic studies and the complex interaction betwee...

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Bibliographic Details
Published in:Canadian Journal of Botany
Main Author: Wallen, D. G.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1974
Subjects:
Online Access:http://dx.doi.org/10.1139/b74-001
http://www.nrcresearchpress.com/doi/pdf/10.1139/b74-001
Description
Summary:The uptake of 14 C-labeled glucose, fructose, and sucrose into Nitella flexilis has been investigated by varying concentrations of sugars, by the use of competition experiments, by the use of inhibitors, and by varying conditions of temperature. The kinetic studies and the complex interaction between the sugars were characteristic of a carrier-mediated system maintained by metabolic energy. Glucose and fructose appear to have different sites of entry though each can influence the other. It is suggested that sucrose is hydrolyzed at the plasmalemma, glucose and fructose then entering the cells.