Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica

Abstract Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU...

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Bibliographic Details
Published in:Antarctic Science
Main Authors: Holovan, Viktoriia, Andriichuk, Olena, Budzanivska, Irena, Zelena, Pavlina, Kondratiuk, Tetiana, Shevchenko, Oleksiy
Other Authors: National Antarctic Scientific Center (NASC) of the Ministry of Education and Science of Ukraine
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 2021
Subjects:
Geology
Ecology, Evolution, Behavior and Systematics
Oceanography
Antarctic
Antarc*
Antarctic Science
Antarctica
Online Access:http://dx.doi.org/10.1017/s0954102021000572
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000572
Description
Summary:Abstract Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU 540) were recovered and characterized. Isolated bacteria belonged to Pseudomonas genus ( Pseudomonas sp., Pseudomonas fluorescens , Pseudomonas putida ) with optimal cultivation temperatures of 16–28°C. These bacteria and previously described Bacillus subtilis FCKU 537 were used for analysing virus-host interactions. Six lytic phages were isolated and named P. fluorescens Antarctic virus 1 (PFAV1), P. fluorescens Antarctic virus 2 (PFAV2), P. fluorescens Antarctic virus 3 (PFAV3), P. putida Antarctic virus 4 (PPAV4), Pseudomonas sp. Antarctic virus 5 (PSAV5) and B. subtilis Antarctic virus 6 (BSAV6) in relation to their natural hosts. According to electron microscopy data, these phages belonged to Caudovirales order. Cross-inoculation demonstrated high specificity of all Antarctic phages, which infected only their initial hosts at moderate temperatures. PFAV2 and PFAV3 phages also infected laboratory Pseudomonas savastanoi and P. fluorescens isolates. This paper adds new data on the occurrence and diversity of viruses and their respective bacterial hosts in soil biotopes of Antarctica.

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