Optimization of phenol degradation by Antarctic bacterium Rhodococcussp.
Abstract This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l -1 . After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of...
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Language: | English |
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Cambridge University Press (CUP)
2020
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Online Access: | http://dx.doi.org/10.1017/s0954102020000358 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102020000358 |
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crcambridgeupr:10.1017/s0954102020000358 2024-09-15T17:44:18+00:00 Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. Tengku-Mazuki, Tengku Athirrah Subramaniam, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima 2020 http://dx.doi.org/10.1017/s0954102020000358 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102020000358 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 32, issue 6, page 486-495 ISSN 0954-1020 1365-2079 journal-article 2020 crcambridgeupr https://doi.org/10.1017/s0954102020000358 2024-07-24T04:03:36Z Abstract This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l -1 . After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l -1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l -1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement ( R 2 = 0.9785) between experimental and predicted values, with less strong but still good agreement ( R 2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Cambridge University Press Antarctic Science 32 6 486 495 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Abstract This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l -1 . After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l -1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l -1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement ( R 2 = 0.9785) between experimental and predicted values, with less strong but still good agreement ( R 2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol. |
format |
Article in Journal/Newspaper |
author |
Tengku-Mazuki, Tengku Athirrah Subramaniam, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima |
spellingShingle |
Tengku-Mazuki, Tengku Athirrah Subramaniam, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. |
author_facet |
Tengku-Mazuki, Tengku Athirrah Subramaniam, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima |
author_sort |
Tengku-Mazuki, Tengku Athirrah |
title |
Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. |
title_short |
Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. |
title_full |
Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. |
title_fullStr |
Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. |
title_full_unstemmed |
Optimization of phenol degradation by Antarctic bacterium Rhodococcussp. |
title_sort |
optimization of phenol degradation by antarctic bacterium rhodococcussp. |
publisher |
Cambridge University Press (CUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1017/s0954102020000358 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102020000358 |
genre |
Antarc* Antarctic Antarctic Science |
genre_facet |
Antarc* Antarctic Antarctic Science |
op_source |
Antarctic Science volume 32, issue 6, page 486-495 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102020000358 |
container_title |
Antarctic Science |
container_volume |
32 |
container_issue |
6 |
container_start_page |
486 |
op_container_end_page |
495 |
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1810491749187977216 |