Optimization of phenol degradation by Antarctic bacterium Rhodococcussp.

• Published in: Antarctic Science
• Main Authors: Tengku-Mazuki, Tengku Athirrah, Subramaniam, Kavilasni, Zakaria, Nur Nadhirah, Convey, Peter, Abdul Khalil, Khalilah, Lee, Gillian Li Yin, Zulkharnain, Azham, Shaharuddin, Noor Azmi, Ahmad, Siti Aqlima
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press (CUP) 2020
• Subjects: Antarctic; The Antarctic; Antarc*; Antarctic Science
• Online Access: http://dx.doi.org/10.1017/s0954102020000358; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102020000358

Abstract This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l -1 . After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l -1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l -1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement ( R 2 = 0.9785) between experimental and predicted values, with less strong but still good agreement ( R 2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol.