Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans

Abstract Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, si...

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Bibliographic Details
Published in:Antarctic Science
Main Authors: Yao, Xinghao, Wang, Tailin, Wang, Huijuan, Liu, Hongwei, Liu, Shenghao, Zhao, Qingang, Chen, Kaoshan, Zhang, Pengying
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 2019
Subjects:
Geology
Ecology, Evolution, Behavior and Systematics
Oceanography
Antarctic
The Antarctic
Antarc*
Antarctic Science
Online Access:http://dx.doi.org/10.1017/s0954102018000470
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102018000470
Description
Summary:Abstract Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, six genes ( Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 and Pn847 ) were identified in the Antarctic moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence alignment and three-dimensional structure analysis revealed the conserved amino acid residues of the enzymes of the chalcone synthase family, including three catalytic residues (Cys 164 , His 303 and Asn 336 ) and two substrate recognition residues (Phe 215 and Phe 265 ). Phylogenetic analysis indicated that PnCHS088, PnCHS444 and PnCHS768 might be chalcone synthase but that Pn021 is more like stilbenecarboxylate synthase. These genes were located at the transition between fungi and advanced plants in the phylogenetic tree. In addition, real-time PCR analysis revealed that the expression profiles of the six P. nutans genes were influenced by diverse abiotic stresses as well as by abscisic acid and methyl jasmonate. The results presented here contribute to the study of the CHS gene family in polar mosses and further reveal the mechanisms underlying the adaptation of mosses to extreme environments.

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