An analysis of the diversity of Haemophilus parainfluenzaein the adult human respiratory tract by genomic DNA fingerprinting
Summary A method for typing Haemophilus species is described, based on the analysis of genomic DNA from Haemophilus parainfluenzae . The DNA was extracted by a rapid method and digested with the restriction enzyme Bam HI to provide a characteristic ‘fingerprint’. The pattern of fragments in the rang...
| Published in: | Epidemiology and Infection |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Cambridge University Press (CUP)
1993
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1017/s0950268800056715 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0950268800056715 |
| Summary: | Summary A method for typing Haemophilus species is described, based on the analysis of genomic DNA from Haemophilus parainfluenzae . The DNA was extracted by a rapid method and digested with the restriction enzyme Bam HI to provide a characteristic ‘fingerprint’. The pattern of fragments in the ranges 1–1·6 kb, 1·6–2 kb and 2–3 kb were used to produce a numerical profile of each isolate. In total 97 isolates were examined; 88 from throat swab material isolated from the 15 members of a British Antarctic Survey base and 9 type strains. Seventy-two of the 88 antarctic isolates were H. parainfluenzae and were found to be very diverse, comprising 41 identifiable strains with up to 5 strains being isolated from a single throat swab sample. There was evidence for both carriage and transmission within the isolated community. The technique provided a highly discriminatory method for characterizing Haemophilus strains which is suitable for epidemiological studies. |
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