XXXI.—Scottish National Antarctic Expedition: A Contribution to the Histology of the Central Nervous System of the Weddell Seal (Leptonychotes weddellii).
The specimens submitted for examination were: ( a ) Portions of the brain (labelled Specimen XXXI.). ( b ) Portions of spinal cord (labelled Specimen XXIV.). Both were in excellent condition as regards fixation and hardening, having been preserved for many years in a fluid composed of formol and 95...
| Published in: | Transactions of the Royal Society of Edinburgh |
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| Main Author: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
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Cambridge University Press (CUP)
1913
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1017/s0080456800013028 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0080456800013028 |
| Summary: | The specimens submitted for examination were: ( a ) Portions of the brain (labelled Specimen XXXI.). ( b ) Portions of spinal cord (labelled Specimen XXIV.). Both were in excellent condition as regards fixation and hardening, having been preserved for many years in a fluid composed of formol and 95 per cent. alcohol (the fluid was also injected into the cerebral vessels). They were, previous to histological examination, submitted to the following processes:— i. Comparatively thin slices were taken from various regions and placed for twenty-four hours in absolute alcohol. ii. Then transferred to acetone for twelve hours. iii. Placed in xylol until permeated. iv. Embedded in paraffin of melting-point 52° C. Sections were then taken with an improved form of the Cambridge rocking microtome, and fixed to slides by means of the albumen method. |
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