Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont
Abstract To facilitate population-genetic studies, we developed simple sequence repeat (SSR) markers and a molecular species identification assay for Peltigera membranacea ( Ascomycota , Peltigerales ), a common ground-dwelling lichen of forest and tundra ecosystems. Additional markers were develope...
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Online Access: | http://dx.doi.org/10.1017/s0024282921000384 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0024282921000384 |
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crcambridgeupr:10.1017/s0024282921000384 2024-03-03T08:49:14+00:00 Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont Werth, Silke Pálsson, Stefán Þór Andrésson, Ólafur S. FP7 People: Marie-Curie Actions 2021 http://dx.doi.org/10.1017/s0024282921000384 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0024282921000384 en eng Cambridge University Press (CUP) http://creativecommons.org/licenses/by/4.0/ The Lichenologist volume 53, issue 6, page 457-465 ISSN 0024-2829 1096-1135 Ecology, Evolution, Behavior and Systematics journal-article 2021 crcambridgeupr https://doi.org/10.1017/s0024282921000384 2024-02-08T08:45:19Z Abstract To facilitate population-genetic studies, we developed simple sequence repeat (SSR) markers and a molecular species identification assay for Peltigera membranacea ( Ascomycota , Peltigerales ), a common ground-dwelling lichen of forest and tundra ecosystems. Additional markers were developed for its Nostoc photobiont. Twenty-one fungal markers for P. membranacea were found to be polymorphic, with the number of alleles ranging from 3–21. Nei's unbiased gene diversity ranged from 0.588 to 0.640 in four significantly structured (F ST = 0.059) mycobiont populations. For the Nostoc photobiont, 14 polymorphic SSR were developed, yielding 4–14 alleles each, with gene diversity ranging from 0.062 to 0.771 in four populations showing substantial population structure (F ST = 0.278). The new markers developed are suitable for population genetic studies of Peltigera membranacea and of its cyanobiont, and at the same time allowed us to distinguish 98.5% of P. membranacea specimens from morphologically similar species of Peltigera . Article in Journal/Newspaper Tundra Cambridge University Press The Lichenologist 53 6 457 465 |
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Cambridge University Press |
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English |
topic |
Ecology, Evolution, Behavior and Systematics |
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Ecology, Evolution, Behavior and Systematics Werth, Silke Pálsson, Stefán Þór Andrésson, Ólafur S. Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont |
topic_facet |
Ecology, Evolution, Behavior and Systematics |
description |
Abstract To facilitate population-genetic studies, we developed simple sequence repeat (SSR) markers and a molecular species identification assay for Peltigera membranacea ( Ascomycota , Peltigerales ), a common ground-dwelling lichen of forest and tundra ecosystems. Additional markers were developed for its Nostoc photobiont. Twenty-one fungal markers for P. membranacea were found to be polymorphic, with the number of alleles ranging from 3–21. Nei's unbiased gene diversity ranged from 0.588 to 0.640 in four significantly structured (F ST = 0.059) mycobiont populations. For the Nostoc photobiont, 14 polymorphic SSR were developed, yielding 4–14 alleles each, with gene diversity ranging from 0.062 to 0.771 in four populations showing substantial population structure (F ST = 0.278). The new markers developed are suitable for population genetic studies of Peltigera membranacea and of its cyanobiont, and at the same time allowed us to distinguish 98.5% of P. membranacea specimens from morphologically similar species of Peltigera . |
author2 |
FP7 People: Marie-Curie Actions |
format |
Article in Journal/Newspaper |
author |
Werth, Silke Pálsson, Stefán Þór Andrésson, Ólafur S. |
author_facet |
Werth, Silke Pálsson, Stefán Þór Andrésson, Ólafur S. |
author_sort |
Werth, Silke |
title |
Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont |
title_short |
Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont |
title_full |
Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont |
title_fullStr |
Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont |
title_full_unstemmed |
Characterization of simple sequence repeat loci for Peltigera membranacea (lichenized Ascomycota ) and its Nostoc photobiont |
title_sort |
characterization of simple sequence repeat loci for peltigera membranacea (lichenized ascomycota ) and its nostoc photobiont |
publisher |
Cambridge University Press (CUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1017/s0024282921000384 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0024282921000384 |
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Tundra |
genre_facet |
Tundra |
op_source |
The Lichenologist volume 53, issue 6, page 457-465 ISSN 0024-2829 1096-1135 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1017/s0024282921000384 |
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The Lichenologist |
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457 |
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