Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation
We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon ( Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using q...
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2012
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Online Access: | http://dx.doi.org/10.1017/s0007114512000396 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512000396 |
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crcambridgeupr:10.1017/s0007114512000396 2024-04-07T07:51:05+00:00 Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation Valente, Luísa M. P. Bower, Neil I. Johnston, Ian A. 2012 http://dx.doi.org/10.1017/s0007114512000396 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512000396 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 108, issue 12, page 2148-2157 ISSN 0007-1145 1475-2662 Nutrition and Dietetics Medicine (miscellaneous) journal-article 2012 crcambridgeupr https://doi.org/10.1017/s0007114512000396 2024-03-08T00:35:39Z We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon ( Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: − 12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1–6 h after feeding and emptied within 48–96 h. IGF-I , MyoD1c , MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1. Article in Journal/Newspaper Atlantic salmon Salmo salar Cambridge University Press British Journal of Nutrition 108 12 2148 2157 |
institution |
Open Polar |
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Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Nutrition and Dietetics Medicine (miscellaneous) |
spellingShingle |
Nutrition and Dietetics Medicine (miscellaneous) Valente, Luísa M. P. Bower, Neil I. Johnston, Ian A. Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
topic_facet |
Nutrition and Dietetics Medicine (miscellaneous) |
description |
We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon ( Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: − 12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1–6 h after feeding and emptied within 48–96 h. IGF-I , MyoD1c , MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1. |
format |
Article in Journal/Newspaper |
author |
Valente, Luísa M. P. Bower, Neil I. Johnston, Ian A. |
author_facet |
Valente, Luísa M. P. Bower, Neil I. Johnston, Ian A. |
author_sort |
Valente, Luísa M. P. |
title |
Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_short |
Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_full |
Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_fullStr |
Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_full_unstemmed |
Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_sort |
postprandial expression of growth-related genes in atlantic salmon ( salmo salar l.) juveniles fasted for 1 week and fed a single meal to satiation |
publisher |
Cambridge University Press (CUP) |
publishDate |
2012 |
url |
http://dx.doi.org/10.1017/s0007114512000396 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512000396 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
British Journal of Nutrition volume 108, issue 12, page 2148-2157 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114512000396 |
container_title |
British Journal of Nutrition |
container_volume |
108 |
container_issue |
12 |
container_start_page |
2148 |
op_container_end_page |
2157 |
_version_ |
1795665939845349376 |