Postprandial expression of growth-related genes in Atlantic salmon ( Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation
We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon ( Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using q...
| Published in: | British Journal of Nutrition |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Cambridge University Press (CUP)
2012
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1017/s0007114512000396 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512000396 |
| Summary: | We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon ( Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: − 12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1–6 h after feeding and emptied within 48–96 h. IGF-I , MyoD1c , MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1. |
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