The vitamin D receptor and its ligand 1α,25-dihydroxyvitamin D3 in Atlantic salmon (Salmo salar)

Seaward migration of Salmo salar is preceded by preparatory physiological adaptations (parr–smolt transformation) to allow for a switch from freshwater (FW) to seawater (SW), which also means a switch in ambient calcium from hypocalcic (<1 mM Ca 2+ ) to the plasma (~1.25 mM Ca 2+ ) and to strongl...

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Bibliographic Details
Published in:Journal of Endocrinology
Main Authors: Lock, E J, Ørnsrud, R, Aksnes, L, Spanings, F A T, Waagbø, R, Flik, G
Format: Article in Journal/Newspaper
Language:unknown
Published: Bioscientifica 2007
Subjects:
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1677/joe-06-0198
https://joe.bioscientifica.com/view/journals/joe/193/3/1930459.xml
https://joe.bioscientifica.com/downloadpdf/journals/joe/193/3/1930459.xml
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Summary:Seaward migration of Salmo salar is preceded by preparatory physiological adaptations (parr–smolt transformation) to allow for a switch from freshwater (FW) to seawater (SW), which also means a switch in ambient calcium from hypocalcic (<1 mM Ca 2+ ) to the plasma (~1.25 mM Ca 2+ ) and to strongly hypercalcic (8–12 mM Ca 2+ ). Uptake, storage (skeleton, scales) and excretion of calcium need careful regulation. In fish, the vitamin D endocrine system plays a rather enigmatic role in calcium physiology. Here, we give direct evidence for calcitriol involvement in SW migration. We report the full sequence of the nuclear vitamin D receptor (sVDR0) and two alternatively spliced variants resulting from intron retention (sVDR1 and sVDR2). In FW parr, SW adapting smolts, and in SW adults, plasma concentrations of 25(OH)D 3 and 24,25(OH) 2 D 3 did not change significantly. Plasma calcitriol concentrations were lowest in FW parr, doubled during smoltification and remained elevated in SW adults. Increased calcitriol coincided with a twofold decrease in sVDR mRNA levels in gill, intestine, and kidney of FW smolts and SW adults, when compared with parr. Clearly, there was a negative feedback and dynamic response of the vitamin D endocrine system during parr–smolt transformation. The onset of these dynamic changes in FW parr warrants a further search for the endocrines that initiate these changes. We speculate that the vitamin D system plays a crucial role in calcium and phosphorus handling in Atlantic salmon.

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