Two variants of the pituitary specific transcription factor Pit-1 in Atlantic salmon

ABSTRACT We have cloned two 1·6 kb cDNAs encoding variants of the POU-type pituitary-specific transcription factor Pit-1 from Atlantic salmon. Sequence comparison with mammalian Pit-1 revealed that the POU domain was highly similar while flanking regions were less conserved. The N-terminal region co...

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Bibliographic Details
Published in:Journal of Molecular Endocrinology
Main Authors: Lorens, J B, Aasland, R, Brunstad, H, Bergh, H, Male, R
Format: Article in Journal/Newspaper
Language:unknown
Published: Bioscientifica 1996
Subjects:
Endocrinology
Molecular Biology
Atlantic salmon
Online Access:http://dx.doi.org/10.1677/jme.0.0170225
https://jme.bioscientifica.com/view/journals/jme/17/3/jme_17_3_006.xml
https://jme.bioscientifica.com/downloadpdf/journals/jme/17/3/jme_17_3_006.xml
Description
Summary:ABSTRACT We have cloned two 1·6 kb cDNAs encoding variants of the POU-type pituitary-specific transcription factor Pit-1 from Atlantic salmon. Sequence comparison with mammalian Pit-1 revealed that the POU domain was highly similar while flanking regions were less conserved. The N-terminal region contained three insertions relative to mammalian Pit-1, one of these corresponded to the insertion found in the alternatively spliced Pit-1a isoform. While two different salmon Pit-1 transcripts were expressed, alternative splicing in the 5′-region did not appear to contribute to further transcript diversity. Both salmon Pit-1 cDNAs encoded 39·5 kDa proteins that specifically bind a consensus Pit-1 recognition sequence in vitro . The salmon Pit-1 proteins also recognized the classical octamer motif; however, a point mutation in the POU homeodomain abolished this interaction.

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