Pseudomonas sp. a Dominant Population of Bacteria in the Cold Water of Mount Sabalan Crater Lake

• Published in: Current Biotechnology
• Main Authors: Ahangari, Hossein, Forouhandeh, Haleh, Ebrahimi, Tahereh, Ebrahimi, Vida, Montazersaheb, Soheila, Tarhriz, Vahideh
• Other Authors: Molecular Medicine Research Center, Tabriz University of Medical Sciences
• Format: Article in Journal/Newspaper
• Language: English
• Published: Bentham Science Publishers Ltd. 2021
• Subjects: Applied Microbiology and Biotechnology; Bioengineering; Biotechnology; Antarctic; Arctic; Crater Lake; Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.2174/2211550110666210917105655; http://eurekaselect.com/article/download/196587; http://www.eurekaselect.com/196587/article

Background: Sabalan (Savalan) Lake is a stable crater lake located at the summit of Sabalan, an inactive stratovolcano and the third highest mountain of Iran. Because of cold weather conditions, the lake is frozen in most months of the year. The biodiversity of microbial flora in this area needs to be explored to find its similarity with the arctic regions’ biodiversity. Objective: The psychrophilic bacterial population of Sabalan (Savalan) Crater Lake was identified. The current research is the first report on aquatic bacterial strains isolation and characterization from Sabalan Lake. Methods: Water sample collections were cultured on four different media, and then colonies were isolated by the plating method. The phylogenetic features of the isolates were scrutinized, and finally, the phenotypic characteristics were investigated using specific culture methods. Results: The results of morphological tests indicated that most isolates were Gram-negative and rod shape, which were able to grow between ˗4 and +37 ºC. According to the phylogenetic analysis, the isolated strains belonged to Pseudomonas, Yersinia, Kocuria, and Micrococcus genera, and about 60% of the isolates belonged to the various species of Pseudomonas as a dominant genus with abounded frequency. In addition, several isolates showed 99% similarity with bacteria, which were previously isolated from Antarctic regions such as Pseudomonas antarctica and Micrococcus antarctica. Conclusion: It can be concluded that the microbial populations of cold areas are the same across geographical distances. In addition, these bacterial strains could be a primitive source of new enzymes for technological applications, such as biosurfactant production.