Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma
A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels ( Anguilla anguilla ) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The a...
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American Society for Microbiology
1979
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crasmicro:10.1128/jvi.30.1.358-364.1979 2023-10-09T21:44:34+02:00 Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma Nagabayashi, Toshihiko Wolf, Ken 1979 http://dx.doi.org/10.1128/jvi.30.1.358-364.1979 https://journals.asm.org/doi/pdf/10.1128/jvi.30.1.358-364.1979 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Virology volume 30, issue 1, page 358-364 ISSN 0022-538X 1098-5514 Virology Insect Science Immunology Microbiology journal-article 1979 crasmicro https://doi.org/10.1128/jvi.30.1.358-364.1979 2023-09-15T05:46:36Z A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels ( Anguilla anguilla ) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm 3 . EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence. Article in Journal/Newspaper Anguilla anguilla ASM Journals (American Society for Microbiology - via Crossref) Journal of Virology 30 1 358 364 |
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ASM Journals (American Society for Microbiology - via Crossref) |
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crasmicro |
language |
English |
topic |
Virology Insect Science Immunology Microbiology |
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Virology Insect Science Immunology Microbiology Nagabayashi, Toshihiko Wolf, Ken Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma |
topic_facet |
Virology Insect Science Immunology Microbiology |
description |
A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels ( Anguilla anguilla ) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm 3 . EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence. |
format |
Article in Journal/Newspaper |
author |
Nagabayashi, Toshihiko Wolf, Ken |
author_facet |
Nagabayashi, Toshihiko Wolf, Ken |
author_sort |
Nagabayashi, Toshihiko |
title |
Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma |
title_short |
Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma |
title_full |
Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma |
title_fullStr |
Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma |
title_full_unstemmed |
Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma |
title_sort |
characterization of ev-2, a virus isolated from european eels ( anguilla anguilla ) with stomatopapilloma |
publisher |
American Society for Microbiology |
publishDate |
1979 |
url |
http://dx.doi.org/10.1128/jvi.30.1.358-364.1979 https://journals.asm.org/doi/pdf/10.1128/jvi.30.1.358-364.1979 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Journal of Virology volume 30, issue 1, page 358-364 ISSN 0022-538X 1098-5514 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/jvi.30.1.358-364.1979 |
container_title |
Journal of Virology |
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30 |
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1 |
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358 |
op_container_end_page |
364 |
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1779311720666234880 |