Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma

• Published in: Journal of Virology
• Main Authors: Nagabayashi, Toshihiko, Wolf, Ken
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 1979
• Subjects: Virology; Insect Science; Immunology; Microbiology; Anguilla anguilla
• Online Access: http://dx.doi.org/10.1128/jvi.30.1.358-364.1979; https://journals.asm.org/doi/pdf/10.1128/jvi.30.1.358-364.1979

A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels ( Anguilla anguilla ) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm 3 . EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence.