Characterization of EV-2, a Virus Isolated from European Eels ( Anguilla anguilla ) with Stomatopapilloma
A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels ( Anguilla anguilla ) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The a...
| Published in: | Journal of Virology |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
American Society for Microbiology
1979
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1128/jvi.30.1.358-364.1979 https://journals.asm.org/doi/pdf/10.1128/jvi.30.1.358-364.1979 |
| Summary: | A virus designated EV-2 has been isolated from external tumor tissue and internal organs of European eels ( Anguilla anguilla ) with stomatopapilloma. It contains RNA and is ether, acid, and temperature labile above 4°C, and concentrated preparations agglutinate chicken and sheep erythrocytes. The addition of actinomycin D during the first 2.75 h of infection inhibits viral replication. As determined in sucrose gradients, the buoyant density of the virus is 1.19 g/cm 3 . EV-2 has a moderately pleomorphic spherical morphology; its diameter ranges from 80 to 140 nm. The virion has narrow, regularly spaced surface projections about 10 nm long. Replication in FHM cells at 15°C shows new infectivity appearing at 10 h postinfection and reaching a plateau at 20 h. Cytopathic effects consist of cell fusion, syncytia, and irregularly rounded cell masses. Viral antigen was detected in the cytoplasm of infected cells by specific immunofluorescence. |
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