A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

ABSTRACT Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity...

Full description

Bibliographic Details
Published in:Journal of Bacteriology
Main Authors: Sun, Boguang, Zhang, Xiao-Hua, Tang, Xuexi, Wang, Shushan, Zhong, Yingbin, Chen, Jixiang, Austin, Brian
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2007
Subjects:
Scophthalmus maximus
Turbot
Online Access:http://dx.doi.org/10.1128/jb.01650-06
https://journals.asm.org/doi/pdf/10.1128/JB.01650-06
id crasmicro:10.1128/jb.01650-06
record_format openpolar
spelling crasmicro:10.1128/jb.01650-06 2024-09-30T14:42:46+00:00 A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus ) Sun, Boguang Zhang, Xiao-Hua Tang, Xuexi Wang, Shushan Zhong, Yingbin Chen, Jixiang Austin, Brian 2007 http://dx.doi.org/10.1128/jb.01650-06 https://journals.asm.org/doi/pdf/10.1128/JB.01650-06 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Journal of Bacteriology volume 189, issue 6, page 2575-2579 ISSN 0021-9193 1098-5530 journal-article 2007 crasmicro https://doi.org/10.1128/jb.01650-06 2024-09-09T04:16:30Z ABSTRACT Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish. Article in Journal/Newspaper Scophthalmus maximus Turbot ASM Journals (American Society for Microbiology) Journal of Bacteriology 189 6 2575 2579
institution Open Polar
collection ASM Journals (American Society for Microbiology)
op_collection_id crasmicro
language English
description ABSTRACT Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.
format Article in Journal/Newspaper
author Sun, Boguang
Zhang, Xiao-Hua
Tang, Xuexi
Wang, Shushan
Zhong, Yingbin
Chen, Jixiang
Austin, Brian
spellingShingle Sun, Boguang
Zhang, Xiao-Hua
Tang, Xuexi
Wang, Shushan
Zhong, Yingbin
Chen, Jixiang
Austin, Brian
A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )
author_facet Sun, Boguang
Zhang, Xiao-Hua
Tang, Xuexi
Wang, Shushan
Zhong, Yingbin
Chen, Jixiang
Austin, Brian
author_sort Sun, Boguang
title A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )
title_short A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )
title_full A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )
title_fullStr A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )
title_full_unstemmed A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )
title_sort single residue change in vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot ( scophthalmus maximus )
publisher American Society for Microbiology
publishDate 2007
url http://dx.doi.org/10.1128/jb.01650-06
https://journals.asm.org/doi/pdf/10.1128/JB.01650-06
genre Scophthalmus maximus
Turbot
genre_facet Scophthalmus maximus
Turbot
op_source Journal of Bacteriology
volume 189, issue 6, page 2575-2579
ISSN 0021-9193 1098-5530
op_rights https://journals.asm.org/non-commercial-tdm-license
op_doi https://doi.org/10.1128/jb.01650-06
container_title Journal of Bacteriology
container_volume 189
container_issue 6
container_start_page 2575
op_container_end_page 2579
_version_ 1811644638559207424

Similar Items