A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

ABSTRACT Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity...

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Bibliographic Details
Published in:Journal of Bacteriology
Main Authors: Sun, Boguang, Zhang, Xiao-Hua, Tang, Xuexi, Wang, Shushan, Zhong, Yingbin, Chen, Jixiang, Austin, Brian
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2007
Subjects:
Molecular Biology
Microbiology
Scophthalmus maximus
Turbot
Online Access:http://dx.doi.org/10.1128/jb.01650-06
https://journals.asm.org/doi/pdf/10.1128/JB.01650-06
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Summary:ABSTRACT Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.

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