Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant α-Amylase

ABSTRACT α-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile α-amylase folds correctly when overexpressed in Escherichia coli , providing the culture temperature is sufficiently low to avoid irreversible denaturation. In t...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Feller, Georges, Le Bussy, Olivier, Gerday, Charles
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 1998
Subjects:
Antarctic
The Antarctic
Antarc*
Online Access:http://dx.doi.org/10.1128/aem.64.3.1163-1165.1998
https://journals.asm.org/doi/pdf/10.1128/AEM.64.3.1163-1165.1998
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Summary:ABSTRACT α-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile α-amylase folds correctly when overexpressed in Escherichia coli , providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18°C.

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