Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted
ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{do...
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Online Access: | http://dx.doi.org/10.1128/aem.02928-07 https://journals.asm.org/doi/pdf/10.1128/AEM.02928-07 |
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crasmicro:10.1128/aem.02928-07 2023-11-05T03:39:28+01:00 Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. 2008 http://dx.doi.org/10.1128/aem.02928-07 https://journals.asm.org/doi/pdf/10.1128/AEM.02928-07 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 74, issue 12, page 3908-3911 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2008 crasmicro https://doi.org/10.1128/aem.02928-07 2023-10-09T15:59:57Z ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(k_{cat}\) \end{document} for biphenyl of 1.1 ± 0.1 s −1 (mean ± standard deviation) at 4°C. In contrast, BPDO LB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDO Cam-1 oxygenase was less than half that of the BPDO LB400 oxygenase. Nevertheless, BPDO Cam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. Article in Journal/Newspaper Arctic ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 74 12 3908 3911 |
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Open Polar |
collection |
ASM Journals (American Society for Microbiology - via Crossref) |
op_collection_id |
crasmicro |
language |
English |
topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
spellingShingle |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted |
topic_facet |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
description |
ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(k_{cat}\) \end{document} for biphenyl of 1.1 ± 0.1 s −1 (mean ± standard deviation) at 4°C. In contrast, BPDO LB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDO Cam-1 oxygenase was less than half that of the BPDO LB400 oxygenase. Nevertheless, BPDO Cam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. |
format |
Article in Journal/Newspaper |
author |
Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. |
author_facet |
Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. |
author_sort |
Master, Emma R. |
title |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted |
title_short |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted |
title_full |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted |
title_fullStr |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted |
title_full_unstemmed |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted |
title_sort |
biphenyl dioxygenase from an arctic isolate is not cold adapted |
publisher |
American Society for Microbiology |
publishDate |
2008 |
url |
http://dx.doi.org/10.1128/aem.02928-07 https://journals.asm.org/doi/pdf/10.1128/AEM.02928-07 |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Applied and Environmental Microbiology volume 74, issue 12, page 3908-3911 ISSN 0099-2240 1098-5336 |
op_rights |
https://journals.asm.org/non-commercial-tdm-license |
op_doi |
https://doi.org/10.1128/aem.02928-07 |
container_title |
Applied and Environmental Microbiology |
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74 |
container_issue |
12 |
container_start_page |
3908 |
op_container_end_page |
3911 |
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1781695289293275136 |