Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted
ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{do...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2008
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Subjects: | |
Online Access: | http://dx.doi.org/10.1128/aem.02928-07 https://journals.asm.org/doi/pdf/10.1128/AEM.02928-07 |
Summary: | ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(k_{cat}\) \end{document} for biphenyl of 1.1 ± 0.1 s −1 (mean ± standard deviation) at 4°C. In contrast, BPDO LB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDO Cam-1 oxygenase was less than half that of the BPDO LB400 oxygenase. Nevertheless, BPDO Cam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. |
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