Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted

ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{do...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Master, Emma R., Agar, Nathalie Y. R., Gómez-Gil, Leticia, Powlowski, Justin B., Mohn, William W., Eltis, Lindsay D.
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2008
Subjects:
Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Arctic
Online Access:http://dx.doi.org/10.1128/aem.02928-07
https://journals.asm.org/doi/pdf/10.1128/AEM.02928-07
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Summary:ABSTRACT Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO Cam-1 ) was purified and found to have an apparent \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(k_{cat}\) \end{document} for biphenyl of 1.1 ± 0.1 s −1 (mean ± standard deviation) at 4°C. In contrast, BPDO LB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDO Cam-1 oxygenase was less than half that of the BPDO LB400 oxygenase. Nevertheless, BPDO Cam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase.

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