Genetic Diversity among Bacillus anthracis Soil Isolates at Fine Geographic Scales

ABSTRACT Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison ( Bison bison ) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from th...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Stratilo, Chad W., Bader, Douglas E.
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2012
Subjects:
Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Wood Buffalo
Wood Buffalo National Park
Bison bison bison
Online Access:http://dx.doi.org/10.1128/aem.01036-12
https://journals.asm.org/doi/pdf/10.1128/AEM.01036-12
Description
Summary:ABSTRACT Environmental samples were collected from carcass sites during and after anthrax outbreaks in 2000 and 2001 in the bison ( Bison bison ) population within Wood Buffalo National Park and the Hook Lake Region north of Wood Buffalo National Park. Bacillus anthracis spores were isolated from these samples and confirmed using phenotypic characterization and real-time PCR. Confirmed B. anthracis isolates were typed using multiple-locus variable-number tandem repeat analysis (MLVA15) and single-nucleotide-repeat analysis (SNRA). B. anthracis isolates split into two clades based on MLVA15, while SNRA allowed some isolates between carcass sites to be distinguished from each other. SNRA polymorphisms were also present within a single carcass site. Some isolates from different carcass sites having the same SNRA type had divergent MLVA types; this finding leads to questions about hierarchical typing methods and the robustness of the fine-scale typing of Bacillus anthracis .

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