Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli

ABSTRACT Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli . The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein produ...

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Published in:Applied and Environmental Microbiology
Main Authors: Narayanan, Niju, Chou, C. Perry
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2009
Subjects:
Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1128/aem.00740-09
https://journals.asm.org/doi/pdf/10.1128/AEM.00740-09
id crasmicro:10.1128/aem.00740-09
record_format openpolar
spelling crasmicro:10.1128/aem.00740-09 2023-11-05T03:34:36+01:00 Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli Narayanan, Niju Chou, C. Perry 2009 http://dx.doi.org/10.1128/aem.00740-09 https://journals.asm.org/doi/pdf/10.1128/AEM.00740-09 en eng American Society for Microbiology https://journals.asm.org/non-commercial-tdm-license Applied and Environmental Microbiology volume 75, issue 16, page 5424-5427 ISSN 0099-2240 1098-5336 Ecology Applied Microbiology and Biotechnology Food Science Biotechnology journal-article 2009 crasmicro https://doi.org/10.1128/aem.00740-09 2023-10-09T16:08:30Z ABSTRACT Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli . The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression. Article in Journal/Newspaper Antarc* Antarctica ASM Journals (American Society for Microbiology - via Crossref) Applied and Environmental Microbiology 75 16 5424 5427
institution Open Polar
collection ASM Journals (American Society for Microbiology - via Crossref)
op_collection_id crasmicro
language English
topic Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
spellingShingle Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Narayanan, Niju
Chou, C. Perry
Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli
topic_facet Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
description ABSTRACT Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli . The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.
format Article in Journal/Newspaper
author Narayanan, Niju
Chou, C. Perry
author_facet Narayanan, Niju
Chou, C. Perry
author_sort Narayanan, Niju
title Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli
title_short Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli
title_full Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli
title_fullStr Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli
title_full_unstemmed Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli
title_sort alleviation of proteolytic sensitivity to enhance recombinant lipase production in escherichia coli
publisher American Society for Microbiology
publishDate 2009
url http://dx.doi.org/10.1128/aem.00740-09
https://journals.asm.org/doi/pdf/10.1128/AEM.00740-09
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Applied and Environmental Microbiology
volume 75, issue 16, page 5424-5427
ISSN 0099-2240 1098-5336
op_rights https://journals.asm.org/non-commercial-tdm-license
op_doi https://doi.org/10.1128/aem.00740-09
container_title Applied and Environmental Microbiology
container_volume 75
container_issue 16
container_start_page 5424
op_container_end_page 5427
_version_ 1781705413004099584

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