Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli

ABSTRACT Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli . The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein produ...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Narayanan, Niju, Chou, C. Perry
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2009
Subjects:
Ecology
Applied Microbiology and Biotechnology
Food Science
Biotechnology
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1128/aem.00740-09
https://journals.asm.org/doi/pdf/10.1128/AEM.00740-09
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Summary:ABSTRACT Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli . The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.

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