Phosphatases in Lake Water: Characterization of Enzymes from Phytoplankton and Zooplankton by Gel Filtration
Sephadex gel filtration was used to characterize phosphomonoesterases in two small lakes in northern Sweden. Two fractions, here termed phosphatase A and phosphatase B, were found both as free enzymes and associated with seston. The activity of phosphatase A was correlated with the presence of algal...
| Published in: | Science |
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| Main Author: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
1976
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1126/science.184531 https://www.science.org/doi/pdf/10.1126/science.184531 |
| Summary: | Sephadex gel filtration was used to characterize phosphomonoesterases in two small lakes in northern Sweden. Two fractions, here termed phosphatase A and phosphatase B, were found both as free enzymes and associated with seston. The activity of phosphatase A was correlated with the presence of algal biomass. Phosphatase B, on the other hand, was derived from zooplankton. Phosphate served as an effective inhibitor of phosphatase A but had no such effect on phosphatase B. Both fractions had p H optima between 6.5 and 7.0. |
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