Friedmanniella endophytica sp. nov., an endophytic actinobacterium isolated from bark of Kandelia candel

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Tuo, Li, Lee, Simon Ming-Yuen, Chen, Li, Hu, Lin, Sun, Cheng-Hang, Li, Fei-Na, Pan, Zhen, Lou, Inchio, Guo, Min
• Format: Other/Unknown Material
• Language: unknown
• Published: 2016
• Subjects: Antarctic; Schumann; Antarc*; Antarctica
• Online Access: https://zenodo.org/record/896142; https://doi.org/10.1099/ijsem.0.001146

A coccus-shaped, non-spore-forming actinobacterium, designated strain 4Q3S-3T, was isolated from surface-sterilized bark of the mangrove plant Kandelia candel collected from Cotai Ecological Zones in Macao, China, and tested by a polyphasic approach to clarify its taxonomic position. This actinobacterium was Gram-stain-positive and aerobic. Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested. Strain 4Q3S-3T grew optimally without NaCl at 28–30 C, pH 7.0–8.0. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain 4Q3S-3T belonged to the genus Friedmanniella and shared the highest 16S rRNA gene sequence similarity with Friedmanniella flava W6T (96.57 %). The DNA G+C content of strain 4Q3S-3T was 69.5 mol%. The cell-wall peptidoglycan contained LL-2,6-diaminopimelic acid, and MK-9(H4) was the predominant menaquinone. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, unidentified glycolipid, amino lipids and two unidentified phospholipids. The predominant fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. Based on phylogenetic, phenotypic and chemotaxonomic data, strain 4Q3S-3T represents a novel species of the genus Friedmanniella, for which the name Friedmanniella endophytica sp. nov. is proposed. The type strain is 4Q3S-3T (=DSM 100723T=CGMCC 4.7307T). The genus Friedmanniella with Friedmanniella antarctica as the type strain was first proposed by Schumann et al. (1997). At the time of preparing this manuscript, the genus contains nine species with validly published names (http://www.bacterio.net/friedmanniella.html#flava; Euzeby, 1997). These species were isolated from different habitats: F. antarctica was isolated from antarctic sandstone (Schumann et al., 1997), Friedmanniella capsulata and Friedmanniella spumicola were reported