Summary: | 1. INTRODUCTION MalaMix is a compiled metabarcoding dataset composed of 451 marine samples collected from a range of depths - from the surface (3m) to deep waters (as far down as 4800m). This dataset covers three ocean layers: the epi- (0-200m – including DCM), meso- (200-1000m) and bathypelagic (1000-4000m). MalaMix combines samples obtained during two oceanographic expeditions with similar sampling strategies: i) the Malaspina-2010 global expedition that produced 263 samples collected between December 2010 and July 2011 from 120 stations distributed along the tropical and subtropical portions (latitudes between 35° N and 40° S) of the Pacific, Atlantic and Indian oceans; and ii) the HotMix trans-Mediterranean cruise that produced 188 samples collected between April and May 2014 in 29 stations distributed along the whole Mediterranean Sea (from -5° W to 33° E) and the adjacent Northeast Atlantic Ocean. MalaMix comprises: a 16S-V4V5 rRNA gene ASV table (MalaMix_16S.csv); an 18S-V4 rRNA gene ASV table (MalaMix_18S.csv); two tables of contextual metadata (MalaMix_EnvData_16S and MalaMix_EnvData_18S) including 6 standardized environmental parameters (temperature [°C], salinity, fluorescence, PO 4 3− [µmol L -1 ], NO 3 − [µmol L -1 ], and SiO 2 [µmol L -1 ]) as well as species taxonomic and phylogenetic diversity metrics a table (MalaMix_FCdata.csv) with flow cytometry microbial counts [cell mL -1 ] and bacterial activity measurements [pmol Leu L -1 h -1 ]; a README file (README_Metadata.csv) describing the meaning and units of each variable column in the metadata tables. The raw DNA sequences are publicly available at the European Nucleotide Archive ( https://www.ebi.ac.uk/ena ) under accession numbers PRJEB23913 [18S rRNA genes] & PRJEB25224 [16S rRNA genes] for the Malaspina surface dataset; PRJEB23771 [18S rRNA genes] & PRJEB45015 [16S rRNA genes] for the Malaspina vertical profiles; PRJEB45011 [16S rRNA genes] & PRJEB45014 [18S rRNA genes] for the Malaspina deep sea dataset; and PRJEB44683 [18S rRNA ...
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