Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers
In this report, we analyze a wild-caught Colias specimen from Kyrgyzstan with unusual wing pattern, which was provisionally identified as a natural hybrid between Colias christophi helialaica Schulte, 1988 and Colias cocandica cocandica Erschoff, 1874 due to its intermediate phenotype. Using one mit...
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ftzenodo:oai:zenodo.org:8353128 2024-09-09T20:08:05+00:00 Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers Shapoval, Nazar A. Krupitsky, Anatoly V. Yakovlev, Roman V. Kir'yanov, Alexander V. Shapoval, Galina N. 2023-09-20 https://doi.org/10.5281/zenodo.8353128 eng eng Zenodo https://zenodo.org/communities/actasibirica https://doi.org/10.5281/zenodo.8353127 https://doi.org/10.5281/zenodo.8353128 oai:zenodo.org:8353128 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Acta Biologica Sibirica, 9, 605-616, (2023-09-20) Central Asia DNA barcodes interspecific hybridisation molecular markers info:eu-repo/semantics/article 2023 ftzenodo https://doi.org/10.5281/zenodo.835312810.5281/zenodo.8353127 2024-07-25T18:56:45Z In this report, we analyze a wild-caught Colias specimen from Kyrgyzstan with unusual wing pattern, which was provisionally identified as a natural hybrid between Colias christophi helialaica Schulte, 1988 and Colias cocandica cocandica Erschoff, 1874 due to its intermediate phenotype. Using one mitochondrial ( COI barcode) and three nuclear ( Ca-ATPase , H3 and CAD gene fragments) markers, we confirm its hybrid origin and demonstrate that this specimen is a hybrid between a female of Colias christophi helialaica and a male of Colias cocandica cocandica . Our study shows that an analysis of mitochondrial and nuclear molecular markers can successfully discriminate natural hybrids in such a taxonomically challenging group of butterflies as the genus Colias . The authors are grateful to Alexander Baryshev (Penza, Russia) for providing material for DNA analysis. The study was supported by the grant of Russian Science Foundation grant No. 22-24-01086 (molecular analysis) and by the state research project No. 122031100272-3 (morphological analysis). Article in Journal/Newspaper Sibirica Zenodo |
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English |
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Central Asia DNA barcodes interspecific hybridisation molecular markers |
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Central Asia DNA barcodes interspecific hybridisation molecular markers Shapoval, Nazar A. Krupitsky, Anatoly V. Yakovlev, Roman V. Kir'yanov, Alexander V. Shapoval, Galina N. Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers |
topic_facet |
Central Asia DNA barcodes interspecific hybridisation molecular markers |
description |
In this report, we analyze a wild-caught Colias specimen from Kyrgyzstan with unusual wing pattern, which was provisionally identified as a natural hybrid between Colias christophi helialaica Schulte, 1988 and Colias cocandica cocandica Erschoff, 1874 due to its intermediate phenotype. Using one mitochondrial ( COI barcode) and three nuclear ( Ca-ATPase , H3 and CAD gene fragments) markers, we confirm its hybrid origin and demonstrate that this specimen is a hybrid between a female of Colias christophi helialaica and a male of Colias cocandica cocandica . Our study shows that an analysis of mitochondrial and nuclear molecular markers can successfully discriminate natural hybrids in such a taxonomically challenging group of butterflies as the genus Colias . The authors are grateful to Alexander Baryshev (Penza, Russia) for providing material for DNA analysis. The study was supported by the grant of Russian Science Foundation grant No. 22-24-01086 (molecular analysis) and by the state research project No. 122031100272-3 (morphological analysis). |
format |
Article in Journal/Newspaper |
author |
Shapoval, Nazar A. Krupitsky, Anatoly V. Yakovlev, Roman V. Kir'yanov, Alexander V. Shapoval, Galina N. |
author_facet |
Shapoval, Nazar A. Krupitsky, Anatoly V. Yakovlev, Roman V. Kir'yanov, Alexander V. Shapoval, Galina N. |
author_sort |
Shapoval, Nazar A. |
title |
Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers |
title_short |
Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers |
title_full |
Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers |
title_fullStr |
Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers |
title_full_unstemmed |
Hybridisation in Colias butterflies (Lepidoptera, Pieridae): identification of natural hybrids using unlinked molecular markers |
title_sort |
hybridisation in colias butterflies (lepidoptera, pieridae): identification of natural hybrids using unlinked molecular markers |
publisher |
Zenodo |
publishDate |
2023 |
url |
https://doi.org/10.5281/zenodo.8353128 |
genre |
Sibirica |
genre_facet |
Sibirica |
op_source |
Acta Biologica Sibirica, 9, 605-616, (2023-09-20) |
op_relation |
https://zenodo.org/communities/actasibirica https://doi.org/10.5281/zenodo.8353127 https://doi.org/10.5281/zenodo.8353128 oai:zenodo.org:8353128 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.835312810.5281/zenodo.8353127 |
_version_ |
1809941779777060864 |