Epidemiological study and Molecular characterization of Toxoplasma gondii infection in Aborted Women in Al-Diwanyah Province, Iraq

• Main Authors: Hind Al-shabani, Marwa sami alwan, Lubna.A.Al-ibrahimi
• Format: Article in Journal/Newspaper
• Language: English
• Published: Zenodo 2023
• Subjects: Toxoplasma gondii; Placenta; ssuRNA gene; Toxoplasmosis; sami
• Online Access: https://doi.org/10.5281/zenodo.8211269

Abstract: Toxoplasma gondii is a protozoan parasite that infected broad range of animals, This study was aimed to detection of T. gondii from aborted placenta of infected women. A total of 65 placenta samples were collected from A borted women from Al-Diwaniya hospital for women and children city, during the period October 2022 until June 2023. The current study showed the infection with the T. gondii parasite was 60.00% (39 of 65 Samples) when examining the placenta microscopic examination by Impression method, and the infection rate was 67.69% (44 of 65 Samples) by Nested-PCR. The results of this study showed that the number and rate of infection in rural was 26 (59.09%) higher than in urban 18 (40.90%) infection was found among the age group 41-50 years 15(34.09 %). The isolation and identification of T. gondii were done using microscopic visualization followed by confirmation using a polymerase chain reaction (PCR) technique targeting the ssuRNA gene, All samples appeared to be contained this gene show one distinct band (313 bp) when electrophoresed on agarose gel. The results of this study indicated that the PCR technique had a high specify in the detection of T. gondii especially this species that encoded to ssuRNA gene isolated from aborted women. Keywords: Toxoplasma gondii, Placenta, ssuRNA gene, Toxoplasmosis. Title: Epidemiological study and Molecular characterization of Toxoplasma gondii infection in Aborted Women in Al-Diwanyah Province, Iraq Author: Hind Al-shabani, Marwa sami alwan, Lubna.A.Al-ibrahimi International Journal of Novel Research in Life Sciences ISSN 2394-966X Vol. 10, Issue 4, July 2023 - August 2023 Page No: 22-25 Novelty Journals Website: www.noveltyjournals.com Published Date: 03-August-2023 DOI: https://doi.org/10.5281/zenodo.8211269 Paper Download Link (Source) https://www.noveltyjournals.com/upload/paper/Epidemiological%20study%20and%20Molecular%20characterization-03082023-6.pdf International Journal of Novel Research in Life Sciences, ISSN 2394-966X, Novelty Journals, ...