Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837
Atlantic salmon ( Salmo salar ) were experimentally infected with Lepeophtheirus salmonis copepodids and aspects of the host’s immune response investigated. Copepodid secretory/excretory product (SEP) produced during early settlement was analysed using fast-protein liquid chromatography (FPLC), sodi...
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ftzenodo:oai:zenodo.org:8106943 2024-09-15T17:56:21+00:00 Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 Dr. Keith Walton 2023-06-28 https://doi.org/10.5281/zenodo.8106943 eng eng Zenodo https://doi.org/10.5281/zenodo.8106943 https://doi.org/10.5281/zenodo.8106942 oai:zenodo.org:8106943 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode International Journal of Indian Science and Research, 2(6), 41 - 51, (2023-06-28) Aquaculture immunosuppressive Endoplasmic reticulum Parasitic antigens info:eu-repo/semantics/article 2023 ftzenodo https://doi.org/10.5281/zenodo.810694310.5281/zenodo.8106942 2024-07-25T18:32:44Z Atlantic salmon ( Salmo salar ) were experimentally infected with Lepeophtheirus salmonis copepodids and aspects of the host’s immune response investigated. Copepodid secretory/excretory product (SEP) produced during early settlement was analysed using fast-protein liquid chromatography (FPLC), sodium dodecyl sulphate (SDS)-electrophoresis and zymography. Following establishment and the appearance of the chalimus stages, the expression of the chemokine interleukin-8 (IL-8) in the heart, spleen, head kidney, fins, liver and pyloric cæca was investigated using real-time (quantitative) PCR (qPCR). Furthermore, the secretions of L. salmonis chalimus were analysed for the presence of the prostanoid PGE 2 using commercially available enzyme-linked immunoassay (EIA) kits. Analysis of copepodid secretory/excretory product suggested that any immunosuppressive component is not proteinaceous in nature. Whilst there was a definite increase in protein concentration of SEP relative to control SEP, further analysis using subtractive chromatographic analysis did not reveal any unique fraction present in either SEP or CSEP that was absent in the other. Interleukin-8 expression levels in tissues changed following L. salmonis infection, with heart and spleen showing significant increases in IL-8 gene expression, whilst the head kidney, fins, liver and pyloric cæca showed no significant increase. The increase in splenic IL-8 expression may be linked to its role as one of the major secondary lymphoid organs. However, this is the first record of increase in IL-8 expression in cardiac tissue. The secretions of L. salmonis chalimus were found to contain quantifiable levels of PGE 2 , albeit in highly variable quantities. This concurs with already published findings for adult L. salmonis (see Fast, et al. 2004). It is proposed that the chalimus states us the PGE 2 to modulate the hosts’ immune response at the site of attachment and feeding. Article in Journal/Newspaper Atlantic salmon Salmo salar Zenodo |
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language |
English |
topic |
Aquaculture immunosuppressive Endoplasmic reticulum Parasitic antigens |
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Aquaculture immunosuppressive Endoplasmic reticulum Parasitic antigens Dr. Keith Walton Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 |
topic_facet |
Aquaculture immunosuppressive Endoplasmic reticulum Parasitic antigens |
description |
Atlantic salmon ( Salmo salar ) were experimentally infected with Lepeophtheirus salmonis copepodids and aspects of the host’s immune response investigated. Copepodid secretory/excretory product (SEP) produced during early settlement was analysed using fast-protein liquid chromatography (FPLC), sodium dodecyl sulphate (SDS)-electrophoresis and zymography. Following establishment and the appearance of the chalimus stages, the expression of the chemokine interleukin-8 (IL-8) in the heart, spleen, head kidney, fins, liver and pyloric cæca was investigated using real-time (quantitative) PCR (qPCR). Furthermore, the secretions of L. salmonis chalimus were analysed for the presence of the prostanoid PGE 2 using commercially available enzyme-linked immunoassay (EIA) kits. Analysis of copepodid secretory/excretory product suggested that any immunosuppressive component is not proteinaceous in nature. Whilst there was a definite increase in protein concentration of SEP relative to control SEP, further analysis using subtractive chromatographic analysis did not reveal any unique fraction present in either SEP or CSEP that was absent in the other. Interleukin-8 expression levels in tissues changed following L. salmonis infection, with heart and spleen showing significant increases in IL-8 gene expression, whilst the head kidney, fins, liver and pyloric cæca showed no significant increase. The increase in splenic IL-8 expression may be linked to its role as one of the major secondary lymphoid organs. However, this is the first record of increase in IL-8 expression in cardiac tissue. The secretions of L. salmonis chalimus were found to contain quantifiable levels of PGE 2 , albeit in highly variable quantities. This concurs with already published findings for adult L. salmonis (see Fast, et al. 2004). It is proposed that the chalimus states us the PGE 2 to modulate the hosts’ immune response at the site of attachment and feeding. |
format |
Article in Journal/Newspaper |
author |
Dr. Keith Walton |
author_facet |
Dr. Keith Walton |
author_sort |
Dr. Keith Walton |
title |
Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 |
title_short |
Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 |
title_full |
Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 |
title_fullStr |
Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 |
title_full_unstemmed |
Aspects of the Atlantic salmon immune response during infection with the salmon louse, Lepeophtheirus salmonis 1837 |
title_sort |
aspects of the atlantic salmon immune response during infection with the salmon louse, lepeophtheirus salmonis 1837 |
publisher |
Zenodo |
publishDate |
2023 |
url |
https://doi.org/10.5281/zenodo.8106943 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
International Journal of Indian Science and Research, 2(6), 41 - 51, (2023-06-28) |
op_relation |
https://doi.org/10.5281/zenodo.8106943 https://doi.org/10.5281/zenodo.8106942 oai:zenodo.org:8106943 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.810694310.5281/zenodo.8106942 |
_version_ |
1810432561642471424 |