| Summary: | True flies (Diptera) are an ecologically important group that play a role in agriculture, public health, and ecosystem functioning. As researchers continue to investigate this order, it is beneficial to link the growing occurrence data to biological traits. However, large-scale ecological trait data are not readily available for fly species. While some databases and datasets include fly data, many ecologically relevant traits for taxa of interest are not included. In this dataset we provide ecological traits (habitat and diet) for fly species of Canada and Greenland having occurrence records on the Barcode of Life Data Systems (BOLD). Trait data were compiled based on literature searches conducted from April 2021 - January 2023 and assigned at the lowest taxonomic level possible. The dataset contains traits for 983 species across 380 genera, 25 subfamilies, and 61 families. This dataset allows for assignment of traits to occurrence data for Diptera species and can be used for further research into the ecology, evolution, and conservation of this order. The dataset is provided in three formats. Only the xlsx file requires Microsoft Excel to open. All files contain the same dataset and information. Funding provided by: Natural Sciences and Engineering Research Council of Canada Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000038 Award Number: Funding provided by: Genome Canada Crossref Funder Registry ID: http://dx.doi.org/10.13039/100008762 Award Number: Funding provided by: Ontario Genomics Crossref Funder Registry ID: http://dx.doi.org/10.13039/100013061 Award Number: Funding provided by: Ontario Ministry of Economic Development, Job Creation and Trade Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100013569 Award Number: Funding provided by: Canada First Research Excellence Fund Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100010785 Award Number:
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