Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles
Gold nanoparticles (AuNPs), owing to their intrinsic plasmonic properties, are widely used in applications ranging from nanotechnology and nanomedicine to catalysis and bioimaging. Capitalising on the ability of AuNPs to generate nanoscale heat upon optical excitation, we designed a nanobiocatalyst...
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ftzenodo:oai:zenodo.org:7528785 2024-09-15T17:42:26+00:00 Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles Giunta, Carolina I Nazemi, Seyed Amirabbas Olesinska, Magdalena Shahgaldian, Patrick 2022-10-21 https://doi.org/10.1039/d2na00605g eng eng Zenodo https://zenodo.org/communities/futurenzyme https://zenodo.org/communities/eu https://doi.org/10.1039/d2na00605g oai:zenodo.org:7528785 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Nanoscale Advances, 5, 81, (2022-10-21) info:eu-repo/semantics/article 2022 ftzenodo https://doi.org/10.1039/d2na00605g 2024-07-26T17:57:52Z Gold nanoparticles (AuNPs), owing to their intrinsic plasmonic properties, are widely used in applications ranging from nanotechnology and nanomedicine to catalysis and bioimaging. Capitalising on the ability of AuNPs to generate nanoscale heat upon optical excitation, we designed a nanobiocatalyst with enhanced cryophilic properties. It consists of gold nanoparticles and enzyme molecules, co-immobilised onto a silica scaffold, and shielded within a nanometre-thin organosilica layer. To produce such a hybrid system, we developed and optimized a synthetic method allowing efficient AuNP covalent immobilisation on the surface of silica particles (SPs). Our procedure allows to reach a dense and homogeneous AuNP surface coverage. After enzyme co-immobilisation, a nanometre-thin organosilica layer was grown on the surface of the SPs. This layer was designed to fulfil the dual function of protecting the enzyme from the surrounding environment and allowing the confinement, at the nanometre scale, of the heat diffusing from the AuNPs after surface plasmon resonance photothermal activation. To establish this proof of concept, we used an industrially relevant lipase enzyme, namely Lipase B from Candida Antarctica (CalB). Herein, we demonstrate the possibility to photothermally activate the so-engineered enzymes at temperatures as low as −10 °C. Article in Journal/Newspaper Antarc* Antarctica Zenodo Nanoscale Advances 5 1 81 87 |
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English |
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Gold nanoparticles (AuNPs), owing to their intrinsic plasmonic properties, are widely used in applications ranging from nanotechnology and nanomedicine to catalysis and bioimaging. Capitalising on the ability of AuNPs to generate nanoscale heat upon optical excitation, we designed a nanobiocatalyst with enhanced cryophilic properties. It consists of gold nanoparticles and enzyme molecules, co-immobilised onto a silica scaffold, and shielded within a nanometre-thin organosilica layer. To produce such a hybrid system, we developed and optimized a synthetic method allowing efficient AuNP covalent immobilisation on the surface of silica particles (SPs). Our procedure allows to reach a dense and homogeneous AuNP surface coverage. After enzyme co-immobilisation, a nanometre-thin organosilica layer was grown on the surface of the SPs. This layer was designed to fulfil the dual function of protecting the enzyme from the surrounding environment and allowing the confinement, at the nanometre scale, of the heat diffusing from the AuNPs after surface plasmon resonance photothermal activation. To establish this proof of concept, we used an industrially relevant lipase enzyme, namely Lipase B from Candida Antarctica (CalB). Herein, we demonstrate the possibility to photothermally activate the so-engineered enzymes at temperatures as low as −10 °C. |
format |
Article in Journal/Newspaper |
author |
Giunta, Carolina I Nazemi, Seyed Amirabbas Olesinska, Magdalena Shahgaldian, Patrick |
spellingShingle |
Giunta, Carolina I Nazemi, Seyed Amirabbas Olesinska, Magdalena Shahgaldian, Patrick Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
author_facet |
Giunta, Carolina I Nazemi, Seyed Amirabbas Olesinska, Magdalena Shahgaldian, Patrick |
author_sort |
Giunta, Carolina I |
title |
Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
title_short |
Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
title_full |
Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
title_fullStr |
Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
title_full_unstemmed |
Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
title_sort |
plasmonic photothermal activation of an organosilica shielded cold-adapted lipase coimmobilised with gold nanoparticles on silica particles |
publisher |
Zenodo |
publishDate |
2022 |
url |
https://doi.org/10.1039/d2na00605g |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Nanoscale Advances, 5, 81, (2022-10-21) |
op_relation |
https://zenodo.org/communities/futurenzyme https://zenodo.org/communities/eu https://doi.org/10.1039/d2na00605g oai:zenodo.org:7528785 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.1039/d2na00605g |
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Nanoscale Advances |
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5 |
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1 |
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81 |
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87 |
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1810489006481211392 |