Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.)
Beaked redfish, dominated by Sebastes mentella, have recently reached record abundance levels in the Gulf of St. Lawrence (GSL) and knowledge of their diet composition is essential to understand the trophic role that these groundfish play in the ecosystem. The objective of the present study was to c...
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ftzenodo:oai:zenodo.org:7401960 2023-06-06T11:59:13+02:00 Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) Brown-Vuillemin, Sarah Bernatchez, Louis Normandeau, Eric Hernandez, Cécilia Chabot, Denis Tremblay, Réjean Sirois, Pascal Nozères, Claude Robert, Dominique 2022-12-05 https://zenodo.org/record/7401960 https://doi.org/10.5061/dryad.37pvmcvpr unknown https://zenodo.org/communities/dryad https://zenodo.org/record/7401960 https://doi.org/10.5061/dryad.37pvmcvpr oai:zenodo.org:7401960 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode Stomach content analysis stomach content DNA info:eu-repo/semantics/other dataset 2022 ftzenodo https://doi.org/10.5061/dryad.37pvmcvpr 2023-04-13T23:16:16Z Beaked redfish, dominated by Sebastes mentella, have recently reached record abundance levels in the Gulf of St. Lawrence (GSL) and knowledge of their diet composition is essential to understand the trophic role that these groundfish play in the ecosystem. The objective of the present study was to compare the performance of the visual examination and DNA metabarcoding of stomach contents of the same individual redfish caught in the estuary and northern Gulf of St. Lawrence. Using a universal metazoan mitochondrial cytochrome c oxidase subunit I (COI) marker, a total of 27 taxonomic sequence matches, 16 at the species level considered as primary prey, were obtained from 185 stomachs with DNA metabarcoding and compared to the 26 prey types, 16 at genus or species level, obtained with stomach content analysis (SCA). While both techniques pointed to a similar definition of diet composition, our results also revealed that the SCA and DNA metabarcoding perform differently among prey categories, both in terms of detectability and taxonomical resolution, as well as in estimated contribution to diet. The use of DNA metabarcoding along with SCA improves the taxonomical resolution of visually determined prey, which supports the concept that both techniques provide useful complementary information that is best used together to gain a maximum level of information on the predator's diet. Microsoft ExcelFunding provided by: Fisheries and Oceans CanadaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000041Award Number: Funding provided by: Resources Aquatic Québec*Crossref Funder Registry ID: Award Number: Funding provided by: Fonds de recherche du Québec – Nature et technologiesCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100003151Award Number: Redfish stomachs were thawed for visual analysis at the Maurice-Lamontagne Institute (Mont-Joli, Canada) and each prey taxon found in the stomach contents was weighed and identified to the lowest taxonomic level possible under a dissecting microscope and ... Dataset Sebastes mentella Zenodo Canada Maurice ENVELOPE(-55.817,-55.817,-63.133,-63.133) Joli ENVELOPE(140.015,140.015,-66.663,-66.663) Mont Joli ENVELOPE(140.015,140.015,-66.663,-66.663) |
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unknown |
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Stomach content analysis stomach content DNA |
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Stomach content analysis stomach content DNA Brown-Vuillemin, Sarah Bernatchez, Louis Normandeau, Eric Hernandez, Cécilia Chabot, Denis Tremblay, Réjean Sirois, Pascal Nozères, Claude Robert, Dominique Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) |
topic_facet |
Stomach content analysis stomach content DNA |
description |
Beaked redfish, dominated by Sebastes mentella, have recently reached record abundance levels in the Gulf of St. Lawrence (GSL) and knowledge of their diet composition is essential to understand the trophic role that these groundfish play in the ecosystem. The objective of the present study was to compare the performance of the visual examination and DNA metabarcoding of stomach contents of the same individual redfish caught in the estuary and northern Gulf of St. Lawrence. Using a universal metazoan mitochondrial cytochrome c oxidase subunit I (COI) marker, a total of 27 taxonomic sequence matches, 16 at the species level considered as primary prey, were obtained from 185 stomachs with DNA metabarcoding and compared to the 26 prey types, 16 at genus or species level, obtained with stomach content analysis (SCA). While both techniques pointed to a similar definition of diet composition, our results also revealed that the SCA and DNA metabarcoding perform differently among prey categories, both in terms of detectability and taxonomical resolution, as well as in estimated contribution to diet. The use of DNA metabarcoding along with SCA improves the taxonomical resolution of visually determined prey, which supports the concept that both techniques provide useful complementary information that is best used together to gain a maximum level of information on the predator's diet. Microsoft ExcelFunding provided by: Fisheries and Oceans CanadaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000041Award Number: Funding provided by: Resources Aquatic Québec*Crossref Funder Registry ID: Award Number: Funding provided by: Fonds de recherche du Québec – Nature et technologiesCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100003151Award Number: Redfish stomachs were thawed for visual analysis at the Maurice-Lamontagne Institute (Mont-Joli, Canada) and each prey taxon found in the stomach contents was weighed and identified to the lowest taxonomic level possible under a dissecting microscope and ... |
format |
Dataset |
author |
Brown-Vuillemin, Sarah Bernatchez, Louis Normandeau, Eric Hernandez, Cécilia Chabot, Denis Tremblay, Réjean Sirois, Pascal Nozères, Claude Robert, Dominique |
author_facet |
Brown-Vuillemin, Sarah Bernatchez, Louis Normandeau, Eric Hernandez, Cécilia Chabot, Denis Tremblay, Réjean Sirois, Pascal Nozères, Claude Robert, Dominique |
author_sort |
Brown-Vuillemin, Sarah |
title |
Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) |
title_short |
Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) |
title_full |
Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) |
title_fullStr |
Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) |
title_full_unstemmed |
Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.) |
title_sort |
data from: dna metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (sebastes sp.) |
publishDate |
2022 |
url |
https://zenodo.org/record/7401960 https://doi.org/10.5061/dryad.37pvmcvpr |
long_lat |
ENVELOPE(-55.817,-55.817,-63.133,-63.133) ENVELOPE(140.015,140.015,-66.663,-66.663) ENVELOPE(140.015,140.015,-66.663,-66.663) |
geographic |
Canada Maurice Joli Mont Joli |
geographic_facet |
Canada Maurice Joli Mont Joli |
genre |
Sebastes mentella |
genre_facet |
Sebastes mentella |
op_relation |
https://zenodo.org/communities/dryad https://zenodo.org/record/7401960 https://doi.org/10.5061/dryad.37pvmcvpr oai:zenodo.org:7401960 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.37pvmcvpr |
_version_ |
1767948984648728576 |