Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway
There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa....
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ftzenodo:oai:zenodo.org:7343350 2024-09-15T18:01:44+00:00 Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine 2022-11-21 https://doi.org/10.5061/dryad.4b8gthtdr unknown Zenodo https://doi.org/10.5281/zenodo.5603319 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.4b8gthtdr oai:zenodo.org:7343350 info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode double-digest restriction site-associated DNA sequencing (ddRAD-seq) Carex bigelowii info:eu-repo/semantics/other 2022 ftzenodo https://doi.org/10.5061/dryad.4b8gthtdr10.5281/zenodo.5603319 2024-07-27T07:13:19Z There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa. We used ddRAD-seq to screen genomic diversity within Carex bigelowii s.lat. focussing on Norway. This species complex is considered a candidate for seeding, but presents considerable morphological, ecological, and genetic variation. The genetic structure of 132 individuals of C . bigelowii s.lat., including C . nigra as an outgroup, was explored using ordinations, clustering analyses, and a genetic barrier algorithm. Two highly divergent clusters were evident, supporting the recognition of two taxonomic units ' C . dacica ' and 'subsp. bigelowii '. Previously defined seed-sourcing regions for C . bigelowii s.lat. did not consider the known taxonomic complexity, and therefore interpreted the overall genetic structure as seed-sourcing regions, not taxa. We estimated genetic neighbourhood sizes within each taxon to be 100-150 km and 300 km, respectively, indicating species-specific delimitations of local seed-sourcing regions. Frequent hybrids, local genetic distinctiveness, and suggested ecotypes add complexity to the discussed seed-sourcing regions. Our results show how genomic screening of diversity and structure in a species complex can alleviate the taxonomic impediment, inform practical questions and legal requirements related to seed-sourcing, and together with traditional taxonomic work provide necessary information for a sound management of biodiversity. Uploaded file "Pop_vs_Sample_vs_CollectorsID" lists the pop_ID, Sample_ID_vcf and the Voucher_ID for all samples. pop_ID is the site number as listed in Table 1 in the publication. Sample_ID_vcf is the individual sample ID as used in the dataset files A and B Voucher_ID is the individual herbarium/DNA-collection identification for each samples, also listed in ... Other/Unknown Material Carex bigelowii Zenodo |
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Open Polar |
collection |
Zenodo |
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ftzenodo |
language |
unknown |
topic |
double-digest restriction site-associated DNA sequencing (ddRAD-seq) Carex bigelowii |
spellingShingle |
double-digest restriction site-associated DNA sequencing (ddRAD-seq) Carex bigelowii Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway |
topic_facet |
double-digest restriction site-associated DNA sequencing (ddRAD-seq) Carex bigelowii |
description |
There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa. We used ddRAD-seq to screen genomic diversity within Carex bigelowii s.lat. focussing on Norway. This species complex is considered a candidate for seeding, but presents considerable morphological, ecological, and genetic variation. The genetic structure of 132 individuals of C . bigelowii s.lat., including C . nigra as an outgroup, was explored using ordinations, clustering analyses, and a genetic barrier algorithm. Two highly divergent clusters were evident, supporting the recognition of two taxonomic units ' C . dacica ' and 'subsp. bigelowii '. Previously defined seed-sourcing regions for C . bigelowii s.lat. did not consider the known taxonomic complexity, and therefore interpreted the overall genetic structure as seed-sourcing regions, not taxa. We estimated genetic neighbourhood sizes within each taxon to be 100-150 km and 300 km, respectively, indicating species-specific delimitations of local seed-sourcing regions. Frequent hybrids, local genetic distinctiveness, and suggested ecotypes add complexity to the discussed seed-sourcing regions. Our results show how genomic screening of diversity and structure in a species complex can alleviate the taxonomic impediment, inform practical questions and legal requirements related to seed-sourcing, and together with traditional taxonomic work provide necessary information for a sound management of biodiversity. Uploaded file "Pop_vs_Sample_vs_CollectorsID" lists the pop_ID, Sample_ID_vcf and the Voucher_ID for all samples. pop_ID is the site number as listed in Table 1 in the publication. Sample_ID_vcf is the individual sample ID as used in the dataset files A and B Voucher_ID is the individual herbarium/DNA-collection identification for each samples, also listed in ... |
format |
Other/Unknown Material |
author |
Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine |
author_facet |
Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine |
author_sort |
Westergaard, Kristine Bakke |
title |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway |
title_short |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway |
title_full |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway |
title_fullStr |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway |
title_full_unstemmed |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway |
title_sort |
using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of carex bigelowii s.lat. in norway |
publisher |
Zenodo |
publishDate |
2022 |
url |
https://doi.org/10.5061/dryad.4b8gthtdr |
genre |
Carex bigelowii |
genre_facet |
Carex bigelowii |
op_relation |
https://doi.org/10.5281/zenodo.5603319 https://zenodo.org/communities/dryad https://doi.org/10.5061/dryad.4b8gthtdr oai:zenodo.org:7343350 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.4b8gthtdr10.5281/zenodo.5603319 |
_version_ |
1810438819263021056 |