PSFcheck ring pattern at various SNR
The imaged PSFcheck pattern consists of a nanometric set of ring-like laser-written structures with a separation of 10 µm between each. The mean FWHM of these patterns was calculated to be 208 nm. A NanoImager-S microscope (ONI, Oxford Nanoimaging), equipped with a 100X 1.4 NA, oil-immersion objecti...
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ftzenodo:oai:zenodo.org:6955019 2023-05-15T17:53:48+02:00 PSFcheck ring pattern at various SNR Damián Martínez 2022-07-20 https://zenodo.org/record/6955019 https://doi.org/10.5281/zenodo.6955019 unknown doi:10.5281/zenodo.6836636 doi:10.21203/rs.3.rs-984659/v1 doi:10.1101/2021.10.17.464398 doi:10.1364/OE.26.021887 doi:10.5281/zenodo.6864455 https://zenodo.org/record/6955019 https://doi.org/10.5281/zenodo.6955019 oai:zenodo.org:6955019 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode info:eu-repo/semantics/other dataset 2022 ftzenodo https://doi.org/10.5281/zenodo.695501910.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.1364/OE.26.02188710.5281/zenodo.6864455 2023-03-11T02:59:58Z The imaged PSFcheck pattern consists of a nanometric set of ring-like laser-written structures with a separation of 10 µm between each. The mean FWHM of these patterns was calculated to be 208 nm. A NanoImager-S microscope (ONI, Oxford Nanoimaging), equipped with a 100X 1.4 NA, oil-immersion objective (Olympus) was used to image the PSFcheck patterns in widefield fluorescence mode. Sample excitation was provided by a 561 nm laser and emission was collected with a 575-616.5 emission filter. A sCMOS sensor (Hamamatsu, Orca-0Flash4.0 V3) was used for image aquisition at 30 fps and 117 nm pixel size. Three files are provided. 'Raw PSFcheck pattern' corresponds to the imaged sample with the intensity displayed as gray levels. 'Photons PSFcheck pattern' displays the intensity as the number of detected photons during image acquisition (see Methods section of the article associated to this dataset). 'ACsN-corrected PSFcheck pattern' contains the ring pattern images after noise correction with the ACsN algorithm [1]. 1. Mandracchia, B., Hua, X., Guo, C. et al. Fast and accurate sCMOS noise correction for fluorescence microscopy. Nat Commun 11, 94 (2020). Dataset Orca Zenodo Olympus ENVELOPE(156.767,156.767,-80.217,-80.217) |
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ftzenodo |
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description |
The imaged PSFcheck pattern consists of a nanometric set of ring-like laser-written structures with a separation of 10 µm between each. The mean FWHM of these patterns was calculated to be 208 nm. A NanoImager-S microscope (ONI, Oxford Nanoimaging), equipped with a 100X 1.4 NA, oil-immersion objective (Olympus) was used to image the PSFcheck patterns in widefield fluorescence mode. Sample excitation was provided by a 561 nm laser and emission was collected with a 575-616.5 emission filter. A sCMOS sensor (Hamamatsu, Orca-0Flash4.0 V3) was used for image aquisition at 30 fps and 117 nm pixel size. Three files are provided. 'Raw PSFcheck pattern' corresponds to the imaged sample with the intensity displayed as gray levels. 'Photons PSFcheck pattern' displays the intensity as the number of detected photons during image acquisition (see Methods section of the article associated to this dataset). 'ACsN-corrected PSFcheck pattern' contains the ring pattern images after noise correction with the ACsN algorithm [1]. 1. Mandracchia, B., Hua, X., Guo, C. et al. Fast and accurate sCMOS noise correction for fluorescence microscopy. Nat Commun 11, 94 (2020). |
format |
Dataset |
author |
Damián Martínez |
spellingShingle |
Damián Martínez PSFcheck ring pattern at various SNR |
author_facet |
Damián Martínez |
author_sort |
Damián Martínez |
title |
PSFcheck ring pattern at various SNR |
title_short |
PSFcheck ring pattern at various SNR |
title_full |
PSFcheck ring pattern at various SNR |
title_fullStr |
PSFcheck ring pattern at various SNR |
title_full_unstemmed |
PSFcheck ring pattern at various SNR |
title_sort |
psfcheck ring pattern at various snr |
publishDate |
2022 |
url |
https://zenodo.org/record/6955019 https://doi.org/10.5281/zenodo.6955019 |
long_lat |
ENVELOPE(156.767,156.767,-80.217,-80.217) |
geographic |
Olympus |
geographic_facet |
Olympus |
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Orca |
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Orca |
op_relation |
doi:10.5281/zenodo.6836636 doi:10.21203/rs.3.rs-984659/v1 doi:10.1101/2021.10.17.464398 doi:10.1364/OE.26.021887 doi:10.5281/zenodo.6864455 https://zenodo.org/record/6955019 https://doi.org/10.5281/zenodo.6955019 oai:zenodo.org:6955019 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.695501910.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.1364/OE.26.02188710.5281/zenodo.6864455 |
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1766161513081470976 |