Volumetric imaging of fluorescently labeled BPAE cells

Using the Nanoimager-S microscope (ONI, Oxford Nanoimaging) with a sCMOS sensor (Hamamatsu, ORCA-Flash4.0 V2), FluoCells™ Prepared Slide #1 (Thermo, #F36924) were imaged with a 100X, 1.4 NA, oil-immersion objective (Olympus). DAPI, Alexa-488 and MitoTracker™ Red excitation was delivered by 405 nm, 4...

Full description

Bibliographic Details
Main Author: Víctor Abonza
Format: Other/Unknown Material
Language:unknown
Published: Zenodo 2022
Subjects:
Orca
Online Access:https://doi.org/10.5281/zenodo.6865066
id ftzenodo:oai:zenodo.org:6865066
record_format openpolar
spelling ftzenodo:oai:zenodo.org:6865066 2024-09-15T18:28:53+00:00 Volumetric imaging of fluorescently labeled BPAE cells Víctor Abonza 2022-07-20 https://doi.org/10.5281/zenodo.6865066 unknown Zenodo https://doi.org/10.5281/zenodo.6836636 https://doi.org/10.21203/rs.3.rs-984659/v1 https://doi.org/10.1101/2021.10.17.464398 https://doi.org/10.5281/zenodo.6865065 https://doi.org/10.5281/zenodo.6865066 oai:zenodo.org:6865066 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode info:eu-repo/semantics/other 2022 ftzenodo https://doi.org/10.5281/zenodo.686506610.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.5281/zenodo.6865065 2024-07-26T05:24:41Z Using the Nanoimager-S microscope (ONI, Oxford Nanoimaging) with a sCMOS sensor (Hamamatsu, ORCA-Flash4.0 V2), FluoCells™ Prepared Slide #1 (Thermo, #F36924) were imaged with a 100X, 1.4 NA, oil-immersion objective (Olympus). DAPI, Alexa-488 and MitoTracker™ Red excitation was delivered by 405 nm, 473 nm and 561 nm lasers, respectively. Light was collected while using two emission filters (1: 525/50; 2: Band 1 575-616.5) and a Channel Splitter dichroic 561 LP. A 3D Z-stack of the sample was acquired for each channel. 22 frames were generated, each separated 50 nm from each other in Z. Other/Unknown Material Orca Zenodo
institution Open Polar
collection Zenodo
op_collection_id ftzenodo
language unknown
description Using the Nanoimager-S microscope (ONI, Oxford Nanoimaging) with a sCMOS sensor (Hamamatsu, ORCA-Flash4.0 V2), FluoCells™ Prepared Slide #1 (Thermo, #F36924) were imaged with a 100X, 1.4 NA, oil-immersion objective (Olympus). DAPI, Alexa-488 and MitoTracker™ Red excitation was delivered by 405 nm, 473 nm and 561 nm lasers, respectively. Light was collected while using two emission filters (1: 525/50; 2: Band 1 575-616.5) and a Channel Splitter dichroic 561 LP. A 3D Z-stack of the sample was acquired for each channel. 22 frames were generated, each separated 50 nm from each other in Z.
format Other/Unknown Material
author Víctor Abonza
spellingShingle Víctor Abonza
Volumetric imaging of fluorescently labeled BPAE cells
author_facet Víctor Abonza
author_sort Víctor Abonza
title Volumetric imaging of fluorescently labeled BPAE cells
title_short Volumetric imaging of fluorescently labeled BPAE cells
title_full Volumetric imaging of fluorescently labeled BPAE cells
title_fullStr Volumetric imaging of fluorescently labeled BPAE cells
title_full_unstemmed Volumetric imaging of fluorescently labeled BPAE cells
title_sort volumetric imaging of fluorescently labeled bpae cells
publisher Zenodo
publishDate 2022
url https://doi.org/10.5281/zenodo.6865066
genre Orca
genre_facet Orca
op_relation https://doi.org/10.5281/zenodo.6836636
https://doi.org/10.21203/rs.3.rs-984659/v1
https://doi.org/10.1101/2021.10.17.464398
https://doi.org/10.5281/zenodo.6865065
https://doi.org/10.5281/zenodo.6865066
oai:zenodo.org:6865066
op_rights info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
op_doi https://doi.org/10.5281/zenodo.686506610.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.5281/zenodo.6865065
_version_ 1810470307962552320

Similar Items