PSFcheck ring pattern
One of the patterns present on a PSFcheck calibration slide consists of a 3D array of small diffraction limited shell features separated 10 µm from each other. The fluorescent thickness of a shell is small compared to the PSF so that the average FWHM across several features was calculated to be of 2...
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2022
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ftzenodo:oai:zenodo.org:6864456 2023-05-15T17:53:41+02:00 PSFcheck ring pattern Damián Martínez 2022-07-20 https://zenodo.org/record/6864456 https://doi.org/10.5281/zenodo.6864456 unknown doi:10.5281/zenodo.6836636 doi:10.21203/rs.3.rs-984659/v1 doi:10.1101/2021.10.17.464398 doi:10.1364/OE.26.021887 doi:10.5281/zenodo.6864455 https://zenodo.org/record/6864456 https://doi.org/10.5281/zenodo.6864456 oai:zenodo.org:6864456 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode info:eu-repo/semantics/other dataset 2022 ftzenodo https://doi.org/10.5281/zenodo.686445610.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.1364/OE.26.02188710.5281/zenodo.6864455 2023-03-11T01:47:36Z One of the patterns present on a PSFcheck calibration slide consists of a 3D array of small diffraction limited shell features separated 10 µm from each other. The fluorescent thickness of a shell is small compared to the PSF so that the average FWHM across several features was calculated to be of 208 nm in a SIM microscope. We imaged this shell pattern using widefield fluorescence excitation on a NanoImager-S (Oxford Nanoimaging Ltd), equipped with a 100X, 1.4 NA, oil-immersion objective (Olympus). The PSFcheck sample was excited with a 561 nm laser and the emitted fluorescence acquired in the Emission Filter 2: Band 1 575-616.5 and recorded on an sCMOS Hamamatsu Orca Flash 4.0 V3. Acquisition time = 33 ms, effective pixel size at object plane = 117 nm. Imaging of the PSFcheck pattern was done once. Dataset Orca Zenodo Olympus ENVELOPE(156.767,156.767,-80.217,-80.217) |
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ftzenodo |
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description |
One of the patterns present on a PSFcheck calibration slide consists of a 3D array of small diffraction limited shell features separated 10 µm from each other. The fluorescent thickness of a shell is small compared to the PSF so that the average FWHM across several features was calculated to be of 208 nm in a SIM microscope. We imaged this shell pattern using widefield fluorescence excitation on a NanoImager-S (Oxford Nanoimaging Ltd), equipped with a 100X, 1.4 NA, oil-immersion objective (Olympus). The PSFcheck sample was excited with a 561 nm laser and the emitted fluorescence acquired in the Emission Filter 2: Band 1 575-616.5 and recorded on an sCMOS Hamamatsu Orca Flash 4.0 V3. Acquisition time = 33 ms, effective pixel size at object plane = 117 nm. Imaging of the PSFcheck pattern was done once. |
format |
Dataset |
author |
Damián Martínez |
spellingShingle |
Damián Martínez PSFcheck ring pattern |
author_facet |
Damián Martínez |
author_sort |
Damián Martínez |
title |
PSFcheck ring pattern |
title_short |
PSFcheck ring pattern |
title_full |
PSFcheck ring pattern |
title_fullStr |
PSFcheck ring pattern |
title_full_unstemmed |
PSFcheck ring pattern |
title_sort |
psfcheck ring pattern |
publishDate |
2022 |
url |
https://zenodo.org/record/6864456 https://doi.org/10.5281/zenodo.6864456 |
long_lat |
ENVELOPE(156.767,156.767,-80.217,-80.217) |
geographic |
Olympus |
geographic_facet |
Olympus |
genre |
Orca |
genre_facet |
Orca |
op_relation |
doi:10.5281/zenodo.6836636 doi:10.21203/rs.3.rs-984659/v1 doi:10.1101/2021.10.17.464398 doi:10.1364/OE.26.021887 doi:10.5281/zenodo.6864455 https://zenodo.org/record/6864456 https://doi.org/10.5281/zenodo.6864456 oai:zenodo.org:6864456 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.686445610.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.1364/OE.26.02188710.5281/zenodo.6864455 |
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