Live-cell imaging of LLC-PK1 cells microtubule dynamics
Stable LLC-PK1 cell line (ATCC:CL-101) was generated and provided by Michael W. Davidson [1,2]. Using a Zeiss Celldiscoverer 7 microscope with a 100X/1.47 NA oil-immersion objective (Plan-Apochromat, Zeiss) and a sCMOS sensor (Hamamatsu, ORCA-Fusion, C15440-20UP), cultured LLC-PK1 cells expressing m...
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ftzenodo:oai:zenodo.org:6850280 2024-09-15T18:28:56+00:00 Live-cell imaging of LLC-PK1 cells microtubule dynamics Alejandro Linares 2022-07-17 https://doi.org/10.5281/zenodo.6850280 unknown Zenodo https://doi.org/10.5281/zenodo.6836636 https://doi.org/10.21203/rs.3.rs-984659/v1 https://doi.org/10.1101/2021.10.17.464398 https://doi.org/10.5281/zenodo.6850279 https://doi.org/10.5281/zenodo.6850280 oai:zenodo.org:6850280 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode info:eu-repo/semantics/other 2022 ftzenodo https://doi.org/10.5281/zenodo.685028010.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.5281/zenodo.6850279 2024-07-25T14:08:05Z Stable LLC-PK1 cell line (ATCC:CL-101) was generated and provided by Michael W. Davidson [1,2]. Using a Zeiss Celldiscoverer 7 microscope with a 100X/1.47 NA oil-immersion objective (Plan-Apochromat, Zeiss) and a sCMOS sensor (Hamamatsu, ORCA-Fusion, C15440-20UP), cultured LLC-PK1 cells expressing mEmerald-EB3 were imaged with a 43 nm pixel size. Exposure time was 100 ms, with each frame being captured every two seconds. During acquisition, temperature and CO2 control was set to 37°C and 5%, respectively. mEmerald-EB3 was excited using a 488 nm laser at 1% power, with a FITC filter for fluorescence collection. Acquisition software ZEN 3.2 (blue edition) was used for the imaging protocol. 1.Rizzo, M. A., Davidson, M. W., & Piston, D. W. (2009). Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Cold Spring Harbor Protocols, 2009(12), pdb-top63. 2.Huang, F., Hartwich, T., Rivera-Molina, F. et al. Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms. Nat Methods 10, 653–658 (2013). Other/Unknown Material Orca Zenodo |
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Stable LLC-PK1 cell line (ATCC:CL-101) was generated and provided by Michael W. Davidson [1,2]. Using a Zeiss Celldiscoverer 7 microscope with a 100X/1.47 NA oil-immersion objective (Plan-Apochromat, Zeiss) and a sCMOS sensor (Hamamatsu, ORCA-Fusion, C15440-20UP), cultured LLC-PK1 cells expressing mEmerald-EB3 were imaged with a 43 nm pixel size. Exposure time was 100 ms, with each frame being captured every two seconds. During acquisition, temperature and CO2 control was set to 37°C and 5%, respectively. mEmerald-EB3 was excited using a 488 nm laser at 1% power, with a FITC filter for fluorescence collection. Acquisition software ZEN 3.2 (blue edition) was used for the imaging protocol. 1.Rizzo, M. A., Davidson, M. W., & Piston, D. W. (2009). Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Cold Spring Harbor Protocols, 2009(12), pdb-top63. 2.Huang, F., Hartwich, T., Rivera-Molina, F. et al. Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms. Nat Methods 10, 653–658 (2013). |
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Other/Unknown Material |
author |
Alejandro Linares |
spellingShingle |
Alejandro Linares Live-cell imaging of LLC-PK1 cells microtubule dynamics |
author_facet |
Alejandro Linares |
author_sort |
Alejandro Linares |
title |
Live-cell imaging of LLC-PK1 cells microtubule dynamics |
title_short |
Live-cell imaging of LLC-PK1 cells microtubule dynamics |
title_full |
Live-cell imaging of LLC-PK1 cells microtubule dynamics |
title_fullStr |
Live-cell imaging of LLC-PK1 cells microtubule dynamics |
title_full_unstemmed |
Live-cell imaging of LLC-PK1 cells microtubule dynamics |
title_sort |
live-cell imaging of llc-pk1 cells microtubule dynamics |
publisher |
Zenodo |
publishDate |
2022 |
url |
https://doi.org/10.5281/zenodo.6850280 |
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op_relation |
https://doi.org/10.5281/zenodo.6836636 https://doi.org/10.21203/rs.3.rs-984659/v1 https://doi.org/10.1101/2021.10.17.464398 https://doi.org/10.5281/zenodo.6850279 https://doi.org/10.5281/zenodo.6850280 oai:zenodo.org:6850280 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.5281/zenodo.685028010.5281/zenodo.683663610.21203/rs.3.rs-984659/v110.1101/2021.10.17.46439810.5281/zenodo.6850279 |
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