Inventory of ancient environmental DNA from sedimentary archives: locations, methods, and target taxa

• Main Authors: Von Eggers, Jordan, Monchamp, Marie-Eve, Capo, Eric, Giguet-Covex, Charline, Spanbauer, Trisha, Heintzman, Peter D
• Other Authors: Barouillet, Cecilia, Brasell, Katie, Duxbury, Lucinda, Epp, Laura S, Fagín, Elena, Ficetola, Gentile Francesco, Harning, David, Ibrahim, Anan, Kisand, Veljo, Maixner, Frank, Mejbel, Hebah, Orsi, William, Pearman, John, Picard, Mailys, Pérez, Vilma, Rouillard, Alexandra, Sarhan, Mohamed S., Stoof-Leichsenring, Kathleen R, Suchora, Magdalena, Thompson, Lucy, Thomson-Laing, Georgia, Thomson-Laing, Jacob, Tsugeki, Narumi, Vandergoes, Marcus, Vuillemin, Aurèle, Vernot, Benjamin, Wesselmann, Marlene, Wood, Susie
• Format: Dataset
• Language: English
• Published: 2022
• Subjects: sedimentary DNA; ancient environmental DNA; paleoecology; paleolimnology; paleooceanography; Canada; permafrost
• Online Access: https://zenodo.org/record/6847522; https://doi.org/10.5281/zenodo.6847522

Locations of sampling sites from sedimentary ancient environmental DNA (aeDNA) studies. aeDNA is DNA that has degraded into short fragments, exhibits post-mortem damage signatures, and is recovered from a non-living tissue, organism, or environmental sample. Here we focus on sedimentary archives with contiguous records such as lake and marine sediments, permafrost, middens, cave sediments, soils, and incidental associated surface sediment samples used to interpret sedimentary archives. Studies span historic and ancient time periods using a variety of DNA-based methods (i.e., metabarcoding, shotgun sequencing, target capture, qPCR, ddPCR, and others) to study taxa from microorganisms to plants and mammals. Funding sources: National Science Foundation: NSF-2011295, EPS-1655726, and NSF-1625040. Natural Sciences and Engineering Research Council of Canada Postdoctoral Fellowship. Severo Ochao Excellence Program Postdoctoral Fellowship: CEX2019-000928-S. Knut & Alice Wallenberg Foundation - Wallenberg Academy Fellowship.