Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis)
Background: Increasing antimicrobial resistance (AMR) is a global threat and wild migratory birds may act as mediators of resistant bacteria across country borders. Our objective was to study extended-spectrum beta-lactamase (ESBL) and plasmid-encoded AmpC (pAmpC) producing Escherichia coli in barna...
Published in: | Open Research Europe |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Zenodo
2021
|
Subjects: | |
Online Access: | https://doi.org/10.12688/openreseurope.13529.1 |
id |
ftzenodo:oai:zenodo.org:5819456 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
Antimicrobial resistance whole genome sequencing extended-spectrum beta-lactamases multidrug resistance migratory birds hybrid sequencing One Health |
spellingShingle |
Antimicrobial resistance whole genome sequencing extended-spectrum beta-lactamases multidrug resistance migratory birds hybrid sequencing One Health Kurittu, Paula Khakipoor, Banafsheh Brouwer, Michael S.M. Heikinheimo, Annamari Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) |
topic_facet |
Antimicrobial resistance whole genome sequencing extended-spectrum beta-lactamases multidrug resistance migratory birds hybrid sequencing One Health |
description |
Background: Increasing antimicrobial resistance (AMR) is a global threat and wild migratory birds may act as mediators of resistant bacteria across country borders. Our objective was to study extended-spectrum beta-lactamase (ESBL) and plasmid-encoded AmpC (pAmpC) producing Escherichia coli in barnacle geese using whole genome sequencing (WGS) and to identify plasmids harboring bla genes. Methods: Barnacle geese feces (n=200) were collected during fall 2017 and spring 2018 from an urban area in Helsinki, Finland. ESBL/AmpC-producing E. coli were recovered from nine samples (4.5%) and isolates were subjected to WGS on both short- and long-read sequencers, enabling hybrid assembly and determination of the genomic location of bla genes. Results: A rare multireplicon IncN+IncR was recovered from one isolate carrying bla CTX-M-1 in addition to aadA2b, lnu(F), and qnrS1. Moreover, rarely detected IncY plasmids in two isolates were found to harbor multiple resistance genes in addition to the human-associated bla CTX-M-15 . Poultry-associated bla CMY-2 was identified from the widely distributed IncI1 and IncK plasmids from four different isolates. One isolate harbored an IncI1 plasmid with bla CTX-M-1 and flor. A chromosomal point mutation in the AmpC promoter was identified in one of the isolates. WGS analysis showed isolates carried multiple resistance and virulence genes and harbored multiple different plasmid replicons in addition to bla-carrying plasmids. Conclusions: Our findings suggest that wild migratory birds serve as a limited source of ESBL/AmpC-producing E. coli and may act as disseminators of the epidemic plasmid types IncI1 and IncK but also rarely detected plasmid types carrying multidrug resistance. Human and livestock-associated ESBL enzyme types were recovered from samples, suggesting a potential for interspecies transmission. WGS offers a thorough method for studying AMR from different sources and should be implemented more widely in the future for AMR surveillance and detection. Understanding ... |
format |
Article in Journal/Newspaper |
author |
Kurittu, Paula Khakipoor, Banafsheh Brouwer, Michael S.M. Heikinheimo, Annamari |
author_facet |
Kurittu, Paula Khakipoor, Banafsheh Brouwer, Michael S.M. Heikinheimo, Annamari |
author_sort |
Kurittu, Paula |
title |
Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) |
title_short |
Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) |
title_full |
Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) |
title_fullStr |
Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) |
title_full_unstemmed |
Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) |
title_sort |
plasmids conferring resistance to extended-spectrum beta-lactamases including a rare incn+incr multireplicon carrying blactx-m-1in escherichia colirecovered from migrating barnacle geese (branta leucopsis) |
publisher |
Zenodo |
publishDate |
2021 |
url |
https://doi.org/10.12688/openreseurope.13529.1 |
genre |
Branta leucopsis |
genre_facet |
Branta leucopsis |
op_source |
Open Research Europe, (2021-05-04) |
op_relation |
https://doi.org/10.1111/1469-0691.12128 https://doi.org/10.1007/s00248-015-0718-0 https://doi.org/10.1186/1471-2164-12-402 https://doi.org/10.1371/journal.pone.0190380 https://doi.org/10.1016/j.vetmic.2020.108766 https://doi.org/10.1093/jac/dkr331 https://doi.org/10.1371/journal.pone.0005958 https://doi.org/10.3201/eid2005.130325 https://doi.org/10.3109/03009734.2014.905663 https://doi.org/10.1016/j.plasmid.2015.04.009 https://doi.org/10.1128/AAC.01707-08 https://doi.org/10.1093/jac/dkq269 https://doi.org/10.1016/j.ijmm.2011.09.003 https://doi.org/10.1016/j.ijmm.2013.02.001 https://doi.org/10.1128/AAC.02412-14 https://doi.org/10.1111/zph.12182 https://doi.org/10.15620/cdc:82532 https://doi.org/10.1128/AAC.00456-06 https://doi.org/10.1186/s12859-018-2336-6 https://doi.org/10.1371/journal.pone.0210686 https://doi.org/10.1016/S1473-3099(19)30273-7 https://doi.org/10.1006/jmbi.1997.1124 https://doi.org/10.1093/jac/dks367 https://doi.org/10.1093/jac/dks387 https://doi.org/10.1016/j.plasmid.2018.09.010 https://doi.org/10.2903/j.efsa.2011.2322 https://doi.org/10.2903/j.efsa.2020.6007 https://doi.org/10.1080/20008686.2017.1300450 https://doi.org/10.1111/j.1469-0691.2012.03850.x https://doi.org/10.1046/j.1365-2958.1998.01144.x https://doi.org/10.1093/jac/dkn470 https://doi.org/10.1371/journal.pone.0053039 https://doi.org/10.1093/jac/dkv444 https://doi.org/10.1128/JCM.02452-13 https://doi.org/10.3402/iee.v6.32112 https://doi.org/10.1089/mdr.2017.0263 https://doi.org/10.1016/S0140-6736(15)00473-0 https://doi.org/10.3389/fmicb.2019.01797 https://doi.org/10.1016/j.jgar.2019.06.007 https://doi.org/10.1128/JCM.03617-13 https://doi.org/10.1128/AEM.02634-07 https://doi.org/10.1038/nature06536 https://doi.org/10.1016/j.psj.2019.10.047 https://doi.org/10.1089/vbz.2015.1935 https://doi.org/10.1186/s12879-018-3245-z https://doi.org/10.1128/JCM.06094-11 https://doi.org/10.1128/JCM.02981-13 https://doi.org/10.1111/j.1469-0691.2011.03497.x https://doi.org/10.3389/fmicb.2019.01687 https://doi.org/10.1111/zph.12141 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.12688/openreseurope.13529.110.1111/1469-0691.1212810.1007/s00248-015-0718-010.1186/1471-2164-12-40210.1371/journal.pone.019038010.1016/j.vetmic.2020.10876610.1093/jac/dkr33110.1371/journal.pone.000595810.3201/eid2005.13032510.3109/03009 |
container_title |
Open Research Europe |
container_volume |
1 |
container_start_page |
46 |
_version_ |
1809904103251247104 |
spelling |
ftzenodo:oai:zenodo.org:5819456 2024-09-09T19:34:37+00:00 Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1in Escherichia colirecovered from migrating barnacle geese (Branta leucopsis) Kurittu, Paula Khakipoor, Banafsheh Brouwer, Michael S.M. Heikinheimo, Annamari 2021-05-04 https://doi.org/10.12688/openreseurope.13529.1 unknown Zenodo https://doi.org/10.1111/1469-0691.12128 https://doi.org/10.1007/s00248-015-0718-0 https://doi.org/10.1186/1471-2164-12-402 https://doi.org/10.1371/journal.pone.0190380 https://doi.org/10.1016/j.vetmic.2020.108766 https://doi.org/10.1093/jac/dkr331 https://doi.org/10.1371/journal.pone.0005958 https://doi.org/10.3201/eid2005.130325 https://doi.org/10.3109/03009734.2014.905663 https://doi.org/10.1016/j.plasmid.2015.04.009 https://doi.org/10.1128/AAC.01707-08 https://doi.org/10.1093/jac/dkq269 https://doi.org/10.1016/j.ijmm.2011.09.003 https://doi.org/10.1016/j.ijmm.2013.02.001 https://doi.org/10.1128/AAC.02412-14 https://doi.org/10.1111/zph.12182 https://doi.org/10.15620/cdc:82532 https://doi.org/10.1128/AAC.00456-06 https://doi.org/10.1186/s12859-018-2336-6 https://doi.org/10.1371/journal.pone.0210686 https://doi.org/10.1016/S1473-3099(19)30273-7 https://doi.org/10.1006/jmbi.1997.1124 https://doi.org/10.1093/jac/dks367 https://doi.org/10.1093/jac/dks387 https://doi.org/10.1016/j.plasmid.2018.09.010 https://doi.org/10.2903/j.efsa.2011.2322 https://doi.org/10.2903/j.efsa.2020.6007 https://doi.org/10.1080/20008686.2017.1300450 https://doi.org/10.1111/j.1469-0691.2012.03850.x https://doi.org/10.1046/j.1365-2958.1998.01144.x https://doi.org/10.1093/jac/dkn470 https://doi.org/10.1371/journal.pone.0053039 https://doi.org/10.1093/jac/dkv444 https://doi.org/10.1128/JCM.02452-13 https://doi.org/10.3402/iee.v6.32112 https://doi.org/10.1089/mdr.2017.0263 https://doi.org/10.1016/S0140-6736(15)00473-0 https://doi.org/10.3389/fmicb.2019.01797 https://doi.org/10.1016/j.jgar.2019.06.007 https://doi.org/10.1128/JCM.03617-13 https://doi.org/10.1128/AEM.02634-07 https://doi.org/10.1038/nature06536 https://doi.org/10.1016/j.psj.2019.10.047 https://doi.org/10.1089/vbz.2015.1935 https://doi.org/10.1186/s12879-018-3245-z https://doi.org/10.1128/JCM.06094-11 https://doi.org/10.1128/JCM.02981-13 https://doi.org/10.1111/j.1469-0691.2011.03497.x https://doi.org/10.3389/fmicb.2019.01687 https://doi.org/10.1111/zph.12141 info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode Open Research Europe, (2021-05-04) Antimicrobial resistance whole genome sequencing extended-spectrum beta-lactamases multidrug resistance migratory birds hybrid sequencing One Health info:eu-repo/semantics/article 2021 ftzenodo https://doi.org/10.12688/openreseurope.13529.110.1111/1469-0691.1212810.1007/s00248-015-0718-010.1186/1471-2164-12-40210.1371/journal.pone.019038010.1016/j.vetmic.2020.10876610.1093/jac/dkr33110.1371/journal.pone.000595810.3201/eid2005.13032510.3109/03009 2024-07-26T09:03:57Z Background: Increasing antimicrobial resistance (AMR) is a global threat and wild migratory birds may act as mediators of resistant bacteria across country borders. Our objective was to study extended-spectrum beta-lactamase (ESBL) and plasmid-encoded AmpC (pAmpC) producing Escherichia coli in barnacle geese using whole genome sequencing (WGS) and to identify plasmids harboring bla genes. Methods: Barnacle geese feces (n=200) were collected during fall 2017 and spring 2018 from an urban area in Helsinki, Finland. ESBL/AmpC-producing E. coli were recovered from nine samples (4.5%) and isolates were subjected to WGS on both short- and long-read sequencers, enabling hybrid assembly and determination of the genomic location of bla genes. Results: A rare multireplicon IncN+IncR was recovered from one isolate carrying bla CTX-M-1 in addition to aadA2b, lnu(F), and qnrS1. Moreover, rarely detected IncY plasmids in two isolates were found to harbor multiple resistance genes in addition to the human-associated bla CTX-M-15 . Poultry-associated bla CMY-2 was identified from the widely distributed IncI1 and IncK plasmids from four different isolates. One isolate harbored an IncI1 plasmid with bla CTX-M-1 and flor. A chromosomal point mutation in the AmpC promoter was identified in one of the isolates. WGS analysis showed isolates carried multiple resistance and virulence genes and harbored multiple different plasmid replicons in addition to bla-carrying plasmids. Conclusions: Our findings suggest that wild migratory birds serve as a limited source of ESBL/AmpC-producing E. coli and may act as disseminators of the epidemic plasmid types IncI1 and IncK but also rarely detected plasmid types carrying multidrug resistance. Human and livestock-associated ESBL enzyme types were recovered from samples, suggesting a potential for interspecies transmission. WGS offers a thorough method for studying AMR from different sources and should be implemented more widely in the future for AMR surveillance and detection. Understanding ... Article in Journal/Newspaper Branta leucopsis Zenodo Open Research Europe 1 46 |