Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae)
Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic mal...
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Online Access: | https://zenodo.org/record/575502 https://doi.org/10.3897/CompCytogen.v9i1.8731 |
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ftzenodo:oai:zenodo.org:575502 2023-05-15T13:36:49+02:00 Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa 2015-02-05 https://zenodo.org/record/575502 https://doi.org/10.3897/CompCytogen.v9i1.8731 unknown https://zenodo.org/communities/biosyslit https://zenodo.org/record/575502 https://doi.org/10.3897/CompCytogen.v9i1.8731 oai:zenodo.org:575502 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode Comparative Cytogenetics 9((1)) 1-15 Animalia Chordata Actinopterygii Perciformes Channichthyidae Chionodraco Chionodraco hamatusAnimalia Sex chromosomes Antarctic fish CHD1 gene info:eu-repo/semantics/article publication-article 2015 ftzenodo https://doi.org/10.3897/CompCytogen.v9i1.8731 2023-03-10T23:12:52Z Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Ch. hamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Ch. hamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action. Article in Journal/Newspaper Antarc* Antarctic Southern Ocean Zenodo Antarctic Southern Ocean The Antarctic The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) Comparative Cytogenetics 9 1 1 15 |
institution |
Open Polar |
collection |
Zenodo |
op_collection_id |
ftzenodo |
language |
unknown |
topic |
Animalia Chordata Actinopterygii Perciformes Channichthyidae Chionodraco Chionodraco hamatusAnimalia Sex chromosomes Antarctic fish CHD1 gene |
spellingShingle |
Animalia Chordata Actinopterygii Perciformes Channichthyidae Chionodraco Chionodraco hamatusAnimalia Sex chromosomes Antarctic fish CHD1 gene Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
topic_facet |
Animalia Chordata Actinopterygii Perciformes Channichthyidae Chionodraco Chionodraco hamatusAnimalia Sex chromosomes Antarctic fish CHD1 gene |
description |
Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Ch. hamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Ch. hamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action. |
format |
Article in Journal/Newspaper |
author |
Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa |
author_facet |
Cocca, Ennio Petraccioli, Agnese Morescalchi, Maria Alessandra Odierna, Gaetano Capriglione, Teresa |
author_sort |
Cocca, Ennio |
title |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_short |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_full |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_fullStr |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_full_unstemmed |
Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae) |
title_sort |
laser microdissection-based analysis of the y sex chromosome of the antarctic fish chionodraco hamatus (notothenioidei, channichthyidae) |
publishDate |
2015 |
url |
https://zenodo.org/record/575502 https://doi.org/10.3897/CompCytogen.v9i1.8731 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Antarctic Southern Ocean The Antarctic The ''Y'' |
geographic_facet |
Antarctic Southern Ocean The Antarctic The ''Y'' |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_source |
Comparative Cytogenetics 9((1)) 1-15 |
op_relation |
https://zenodo.org/communities/biosyslit https://zenodo.org/record/575502 https://doi.org/10.3897/CompCytogen.v9i1.8731 oai:zenodo.org:575502 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.3897/CompCytogen.v9i1.8731 |
container_title |
Comparative Cytogenetics |
container_volume |
9 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
15 |
_version_ |
1766084532638842880 |