Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae)

Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic mal...

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Bibliographic Details
Published in:Comparative Cytogenetics
Main Authors: Cocca, Ennio, Petraccioli, Agnese, Morescalchi, Maria Alessandra, Odierna, Gaetano, Capriglione, Teresa
Format: Article in Journal/Newspaper
Language:unknown
Published: 2015
Subjects:
Animalia
Chordata
Actinopterygii
Perciformes
Channichthyidae
Chionodraco
Chionodraco hamatusAnimalia
Sex chromosomes
Antarctic fish
CHD1 gene
Antarctic
Southern Ocean
The Antarctic
The ''Y''
Antarc*
Online Access:https://zenodo.org/record/575502
https://doi.org/10.3897/CompCytogen.v9i1.8731
Description
Summary:Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Ch. hamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Ch. hamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action.

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