Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring
Balancing selection is one of the mechanisms which has been proposed to explain the maintenance of genetic diversity in species across generations. For species with large populations and complex life histories, however, heterogeneous selection pressures may create a scenario in which the net effects...
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ftzenodo:oai:zenodo.org:5182847 2023-05-15T15:58:18+02:00 Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring Durland, Evan De Wit, Pierre Langdon, Chris 2021-10-20 https://zenodo.org/record/5182847 https://doi.org/10.5061/dryad.2z34tmpn5 unknown doi:10.1098/rspb.2020.3223 https://zenodo.org/communities/dryad https://zenodo.org/record/5182847 https://doi.org/10.5061/dryad.2z34tmpn5 oai:zenodo.org:5182847 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode Balancing selection Oysters genetics PoolSeq larval development info:eu-repo/semantics/other dataset 2021 ftzenodo https://doi.org/10.5061/dryad.2z34tmpn510.1098/rspb.2020.3223 2023-03-10T13:51:54Z Balancing selection is one of the mechanisms which has been proposed to explain the maintenance of genetic diversity in species across generations. For species with large populations and complex life histories, however, heterogeneous selection pressures may create a scenario in which the net effects of selection are balanced across developmental stages. With replicated cultures and a pooled sequencing approach, we show that genotype-dependent mortality in larvae of the Pacific oyster (Crassostrea gigas) is largely temporally dynamic and inconsistently in favor of a single genotype or allelic variant at each locus. Overall, the patterns of genetic change we observe to be taking place are more complex than what would be expected under classical examples of additive or dominant genetic interactions. They are also not easily explained by our current understanding of the effects of genetic load. Collectively, temporally heterogeneous selection pressures across different larval developmental stages may act to maintain genetic diversity in oysters, while also inherently sheltering genetic load within populations. Please see manuscript for bioinformatic approaches and associated github page: GitHub - E-Durland/oyster-poolseq: Methods for library preparation and bioinformatic analysis of pooled DNA data from larval oyster samples Please see README file for description of the files. Funding provided by: Oregon Sea Grant, Oregon State UniversityCrossref Funder Registry ID: http://dx.doi.org/10.13039/100005798Award Number: NA14OAR4170064 (R/SAQ-20)Funding provided by: Agricultural Research ServiceCrossref Funder Registry ID: http://dx.doi.org/10.13039/100005798Award Number: 2072-31000-004-00D (CRIS Project Number) Pooled samples of oyster larvae (n=200-3000) were collected at various points during development (days 0,2,6,10,14,22). DNA was extracted in bulk, converted to 2bRAD libraries (BCGI enzyme) and sequenced on an Illumina Hiseq (SE 50bp reads). Dataset Crassostrea gigas Pacific oyster Cris Zenodo Pacific |
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Balancing selection Oysters genetics PoolSeq larval development |
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Balancing selection Oysters genetics PoolSeq larval development Durland, Evan De Wit, Pierre Langdon, Chris Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring |
topic_facet |
Balancing selection Oysters genetics PoolSeq larval development |
description |
Balancing selection is one of the mechanisms which has been proposed to explain the maintenance of genetic diversity in species across generations. For species with large populations and complex life histories, however, heterogeneous selection pressures may create a scenario in which the net effects of selection are balanced across developmental stages. With replicated cultures and a pooled sequencing approach, we show that genotype-dependent mortality in larvae of the Pacific oyster (Crassostrea gigas) is largely temporally dynamic and inconsistently in favor of a single genotype or allelic variant at each locus. Overall, the patterns of genetic change we observe to be taking place are more complex than what would be expected under classical examples of additive or dominant genetic interactions. They are also not easily explained by our current understanding of the effects of genetic load. Collectively, temporally heterogeneous selection pressures across different larval developmental stages may act to maintain genetic diversity in oysters, while also inherently sheltering genetic load within populations. Please see manuscript for bioinformatic approaches and associated github page: GitHub - E-Durland/oyster-poolseq: Methods for library preparation and bioinformatic analysis of pooled DNA data from larval oyster samples Please see README file for description of the files. Funding provided by: Oregon Sea Grant, Oregon State UniversityCrossref Funder Registry ID: http://dx.doi.org/10.13039/100005798Award Number: NA14OAR4170064 (R/SAQ-20)Funding provided by: Agricultural Research ServiceCrossref Funder Registry ID: http://dx.doi.org/10.13039/100005798Award Number: 2072-31000-004-00D (CRIS Project Number) Pooled samples of oyster larvae (n=200-3000) were collected at various points during development (days 0,2,6,10,14,22). DNA was extracted in bulk, converted to 2bRAD libraries (BCGI enzyme) and sequenced on an Illumina Hiseq (SE 50bp reads). |
format |
Dataset |
author |
Durland, Evan De Wit, Pierre Langdon, Chris |
author_facet |
Durland, Evan De Wit, Pierre Langdon, Chris |
author_sort |
Durland, Evan |
title |
Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring |
title_short |
Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring |
title_full |
Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring |
title_fullStr |
Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring |
title_full_unstemmed |
Temporally-balanced selection during development of larval Pacific oysters (Crassostrea gigas) inherently preserves genetic diversity within offspring |
title_sort |
temporally-balanced selection during development of larval pacific oysters (crassostrea gigas) inherently preserves genetic diversity within offspring |
publishDate |
2021 |
url |
https://zenodo.org/record/5182847 https://doi.org/10.5061/dryad.2z34tmpn5 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster Cris |
genre_facet |
Crassostrea gigas Pacific oyster Cris |
op_relation |
doi:10.1098/rspb.2020.3223 https://zenodo.org/communities/dryad https://zenodo.org/record/5182847 https://doi.org/10.5061/dryad.2z34tmpn5 oai:zenodo.org:5182847 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/legalcode |
op_doi |
https://doi.org/10.5061/dryad.2z34tmpn510.1098/rspb.2020.3223 |
_version_ |
1766394031735046144 |