Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia

• Main Authors: Ettinger, Cassandra, Wilkins, Laetitia, Jospin, Guillaume, Eisen, Jonathan
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Arkashin Shurf; Zavarzin Spring; Uzon Caldera; Kamchatka; Russia; global microbial diversity; Metagenome-assembled genomes (MAG's); Burgess; Kamchatka Peninsula
• Online Access: https://zenodo.org/record/5008727; https://doi.org/10.25338/B8N01R

Culture-independent methods have contributed substantially to our understanding of global microbial diversity. Recently developed algorithms to construct whole genomes from environmental samples have further refined, corrected and revolutionized understanding of the tree of life. Here, we assembled draft metagenome-assembled genomes (MAGs) from environmental DNA extracted from two hot springs within an active volcanic ecosystem on the Kamchatka peninsula, Russia. This hydrothermal system has been intensively studied previously with regard to geochemistry, chemoautotrophy, microbial isolation, and microbial diversity. We assembled genomes of bacteria and archaea using DNA that had previously been characterized via 16S rRNA gene clone libraries. We recovered 36 MAGs, 29 of medium to high quality, and inferred their placement in a phylogenetic tree consisting of 3,240 publicly available microbial genomes. We highlight MAGs that were taxonomically assigned to groups previously underrepresented in available genome data. This includes several archaea (Korarchaeota, Bathyarchaeota and Aciduliprofundum) and one potentially new species within the bacterial genus Sulfurihydrogenibium. Putative functions in both pools were compared and are discussed in the context of their diverging geochemistry. This study adds comprehensive information about phylogenetic diversity and functional potential within two hot springs in the caldera of Kamchatka. Please see included CSV which includes a key to identify draft MAG's as well as a summary of individual MAG assembly statistics, quality measurements and taxonomic inferences. In this study we focus on two hydrothermal pools, Arkashin Shurf and Zavarzin Spring, in Uzon Caldera, Kamchatka, Russia that were previously characterized using 16S ribosomal RNA sequencing and geochemical analysis undertaken in Burgess et al. 2012 (DOI:10.1007/s00248-011-9979-4). First we produced metagenome-assembled genomes (MAGs) and then we characterized and compared them between the two pools. Raw ...